华北农学报2018，Vol.33Issue(2)：139-148,10.DOI:10.7668/hbnxb.2018.02.020
苏打盐碱胁迫下粳稻芽期耐盐碱相关性状与SSR标记关联分析
Association Analysis of Saline-alkali Related Traits and SSR Markers in Japonica Rice under Soda Saline-alkali Stress
摘要
Abstract
In order to identify the elite allelic variation of salt-and-alkali-tolerance traits that affect the budding stage of Japonica rice and the related carriers for the improvement of saline-alkali potential of cultivars,the natural group consists of 176 Japonica rice from different geographical origins was used for research materials. For direct seeding of rice planting methods,the medium soda salt alkaline soil in Daqing Area of Heilongjiang Province was used for bud stress treatment. The shoot Na+concentration(SNC),shoot K+concentration(SKC),shoot Na+/K+(SNK) of 13 d and 18 d,and salinization damage grade (SDG) of 18 d from the aboveground part of the budding stage were identified,which were related to the saline-alkaline tolerance of Japonica rice. At the same time,154 SSR marker genotypes of 176 cultivars were identified,STRUCTURE 2.2 software was used to analyze the popula-tion structure and linkage disequilibrium,the correlation analysis between the marker and the trait was carried out by the general linear model(GLM) and the mixed linear model(MLM) of TASSEL 3.0 software,and the superior mutation and related vector materials. The results showed that the natural population was divided into three sub-groups,and each subgroup had different degrees of linkage disequilibrium. In the process of correlation analysis,a total of 13 locis was found that was significantly associated with salt-alkaline resistance in Japonica rice at the bud stage,of which 8 locis were detected simultaneously under the two model conditions of TASSEL 3.0 software further identification at 8 locis resulted in 24 excellent allelic variations. By comparing the maps,it was found that among the eight sites simultaneously found to be significantly associated with salinity-tolerance traits,seven were previously reported sites under the two model conditions,whereas RM1219 was not found in previous studies. Reported that there was a significant correlation between RM1219 and three salt-tolerant traits,which may be a new site associated with salt-alkaline resistance in Japonica rice at the budding stage.关键词
粳稻/耐盐碱/关联分析/芽期Key words
Japonica rice/Saline-alkaline tolerance/Association analysis/Budding stage分类
生物科学引用本文复制引用
李宪伟,郑洪亮,赵宏伟,王敬国,刘化龙,孙健,李宁,雷蕾,邹德堂..苏打盐碱胁迫下粳稻芽期耐盐碱相关性状与SSR标记关联分析[J].华北农学报,2018,33(2):139-148,10.基金项目
国家自然科学基金项目(31601377) （31601377）
黑龙江省重大科技招标基金项目(GA14B102-02) （GA14B102-02）
国家重点研发计划项目(2017YFD0100503-2) （2017YFD0100503-2）
