华北农学报2018，Vol.33Issue(2)：149-156,8.DOI:10.7668/hbnxb.2018.02.021
PCV2逃逸宿主天然免疫的分子机制研究
Molecular Mechanisms of PCV2 Escape from Innate Immunity
摘要
Abstract
To reveal the relationship between PCV2 infection and innate immune response as well as its molecu-lar mechanism,a strain of PCV2 was adapted to PK-15 cell and PAMs,and relative fluorescence quantitative PCR as-say was developed to assess the impacts of PCV2 infection,cytokines,antigen processing machineries and adaptor mol-ecules were investigated.The results indicated that the IFN-β production was significantly suppressed during PCV2 in-fection in PK-15.Using luciferase reporter assays,the result showed that PCV2 infection impairs IRF3/NF-κB media-ted IFN-β production. The different PCV2 expression vectors (ORF1,ORF2,or ORF3) were cotransfected with the report plasmid of the IFN-β-Luc,IRF3-Luc,NF-κB-Luc or AP-1-Luc,respectively,the luciferase reporter assays showed that the PCV2 ORF1 enhanced activation of IFN-β promoter,while IFN-β promoter was impeded by ORF2,no impact was detected by ORF3.The results showed that the inhibition of innate immunity in PCV2 could be contribu-ted to PCV2 ORF2 by downregulating of IFN production by regulating transcription factor of IRF3 and NF-кB.关键词
猪圆环病毒2型/ORF1/ORF2/天然免疫/IFN-βKey words
PCV2/ORF1/ORF2/Innate immunity/IFN-β分类
农业科技引用本文复制引用
张蕾,代松宝,张丽琳,时培殿,王家顺,任婕,黄金海..PCV2逃逸宿主天然免疫的分子机制研究[J].华北农学报,2018,33(2):149-156,8.基金项目
天津市科委项目(16YFZCNC00640) （16YFZCNC00640）
自然科学基金重点项目(17JCZDJC33900) （17JCZDJC33900）
天津大学大学生创新训练项目(201510056278) （201510056278）
