河南农业大学学报2018，Vol.52Issue(2)：212-216,5.
鸽副黏病毒经鸡胚传代引起毒力和F基因序列变化的研究
Passaging of a pigeon paramyxovirus in chicken embryo results in virus virulence and F gene sequence
摘要
Abstract
In order to study the virulence and F gene sequence of ND167 in 10-day-old specific-pathogen-free (SPF) chicken embryos,the virus was passaged by chicken embryo Limit dilution method.Allantonic fluid with the highest dilution rate and hemagglutiantion titer was chosen for further Limit dilution.We purified ND167 for 10 generations,and the MDT,ICPI and F gene sequences of the 1st,5th and 10th generation were determined.The results showed that the amino acid of F protein cleavage site in ND167 was not changed and no significant mutation was found during the purification,but the virulence was gradually increased from medium virulent strain to highly virulent strain.This result suggests that the researchers should determine PPMV virulence based on animal experiments and be alert to the increased virulence of PPMV in the flocks caused by repeated passage.关键词
鸽副黏病毒/传代/毒力/F基因序列Key words
pigeon paramyxovirus/passage in chicken embryo/virulence/F gene sequence分类
农业科技引用本文复制引用
吴双,朱善元,郭长明,左伟勇,王永娟,秦枫,洪伟鸣,王安平..鸽副黏病毒经鸡胚传代引起毒力和F基因序列变化的研究[J].河南农业大学学报,2018,52(2):212-216,5.基金项目
国家自然科学基金项目(31440083) （31440083）
江苏省青蓝工程项目(00000216047) （00000216047）
江苏省六大人才高峰项目(NY-009) （NY-009）
江苏农牧科技职业学院科研项目(NSFPT201501,NSFPT201711) （NSFPT201501,NSFPT201711）
江苏省农业自主创新资金项目[cx(16)1055]. （16）
