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漆酶对纤维素酶催化作用的影响

王旭 侯华伟 司玲玉 杨森 陈红歌

河南农业大学学报2018,Vol.52Issue(2):244-248,253,6.
河南农业大学学报2018,Vol.52Issue(2):244-248,253,6.

漆酶对纤维素酶催化作用的影响

Effect of laccase on catalytic efficiency of cellulase

王旭 1侯华伟 1司玲玉 1杨森 1陈红歌1

作者信息

  • 1. 河南农业大学生命科学学院,河南 郑州 450002
  • 折叠

摘要

Abstract

In order to investigate the effect of laccase on catalytic efficiency of cellulase,a comparative analysis of sugar yield from cellulosic hydrolysate of filter paper and corn stover using different addition level of the pure laccase(Sigma) was performed in this study.The results indicated that the addition of lactase had no significant effect on the sugar yield in the filter paper hydrolysis system which contained cellulase.However,the addition of lactase could decrease the sugar yield (from 30.40% to 23.87%) obviously in the corn stover hydrolysis system (preincubating laccase and corn stover together at 37℃ for 24 h).A negative correlation between the sugar yield and the addition of lactase in the corn stover hydrolysis system was also observed.Hence,laccase had played an inhibitory role in cellulase catalysis in the corn stover hydrolysis system.

关键词

漆酶/纤维素酶/糖得率/玉米秸秆

Key words

laccase/cellulase/sugar yield/corn stover

分类

生物科学

引用本文复制引用

王旭,侯华伟,司玲玉,杨森,陈红歌..漆酶对纤维素酶催化作用的影响[J].河南农业大学学报,2018,52(2):244-248,253,6.

基金项目

公益性行业(农业)科研专项(201503134) (农业)

河南农业大学学报

OA北大核心CSCDCSTPCD

1000-2340

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