华南农业大学学报2018，Vol.39Issue(3)：1-5,5.DOI:10.7671/j.issn.1001-411X.2018.03.001
猪圆环病毒2型重组衣壳蛋白在昆虫细胞中的表达及免疫原性分析
Expression and immunogenicity of recombinant capsid protein of porcine circovirus type 2 in insect cells
摘要
Abstract
[Objective]To express the capsid protein (Cap) of porcine circovirus type 2 (PCV2) in insect cells and analyze the immunogenicity of recombinant protein.[Method]The established recombinant bacmid containing fused ORF2 gene(Bacmid-ORF2)was transfected into insect cells for protein expression test,and then the purified *Cap and pcDNA3.1-ORF2 plasmid were immunized mice respectively to compare their immunological effects.[Result]The Cap structural protein encoded by PCV2-ORF2 gene was correctly expressed in insect cells, and the relative molecular mass was about 32 000. *Cap could be recognized by PCV2 positive serum and had good antigenicity. The immue effect of *Cap group was better than that of pcDNA3.1-ORF2 group in both cellular immunity and humoral immunity levels.[Conclusion]The recombinant protein*Cap can replace the whole virus and may have potential application values of PCV2 vaccinum. These results lay the foundation for the study of molecular diagnosis, subunit vaccine development and molecular biology research of PCV2.关键词
猪圆环病毒2型/衣壳蛋白/重组杆状病毒质粒/表达/免疫Key words
porcine circovirus type 2/capsid protein/recombinant bacmid/expression/immune分类
农业科技引用本文复制引用
徐铮,马晓莉,林彦星,董建国,王磊,刘燕玲,刘清神,宋长绪..猪圆环病毒2型重组衣壳蛋白在昆虫细胞中的表达及免疫原性分析[J].华南农业大学学报,2018,39(3):1-5,5.基金项目
"十二五"农村领域国家科技计划(2015BAD12B02-5) （2015BAD12B02-5）
现代农业产业共性技术创新团队项目(2016LM2150) （2016LM2150）
广东省科技计划(2016A020212021,2015B020230004) （2016A020212021,2015B020230004）
广州市科技计划(201508020062) （201508020062）
