华南农业大学学报2018，Vol.39Issue(3)：6-12,7.DOI:10.7671/j.issn.1001-411X.2018.03.002
腹泻犬源大肠埃希菌耐药性以及整合子–基因盒检测研究
Antimicrobial resistance and integron-gene cassette detection of Escherichia coli isolates from diarrhea dogs
摘要
Abstract
[Objective]To investigate the antimicrobial resistance of Escherichia coli from diarrhea dogs and the distribution of integron-gene cassettes in E.coli.[Method]The Kirby-Bauer disc diffusion method was used to test the susceptibility of 30 strains of E.coli against 19 antimicrobial agents.PCR was used to detect the integrase genes(class I,II,III)in E.coli isolates.We further detected if E.coli isolates carrying integrase genes also carried the sul1,qacEΔ1 genes and gene cassettes in variable regions.[Result]Thirty isolates showed different levels of resistance to 19 antimicrobial agents with 18 kinds of resistant spectrum.E.coli isolates had high resistance to amoxicillin, ampicillin, tetracycline and doxycycline with drug resistance rates of 90.00%, 83.33%, 66.67% and 63.33%, respectively, while had low resistance to the other antimicrobials with drug resistance rates below 14.00%.Eleven out of 30 isolates were found to carry class I integrase,sul1 and qacEΔ1 genes, and class II or class III integrase gene was not detected. Among the isolates carrying class I integrase gene,the dfrA12+orfF+aadA2 gene cassette of 1 879 bp length was successfully amplified from two isolates.[Conclusion]E.coli isolates from diarrhea dogs show different levels of resistance to antimicrobial agents. Antimicrobial resistance that mediated by gene cassettes is partially related to the resistant phenotype of E.coli. Class I integron shows a certain relationship with the antimicrobial resistance of E.coli.关键词
犬/大肠埃希菌/耐药性/K-B纸片法/整合子–基因盒Key words
dog/Escherichia coli/resistance/Kirby-Bauer disc diffusion method/integron-gene cassette分类
农业科技引用本文复制引用
魏斌,杨亭玉,彭广能,钟志军,欧红萍,严光文,田一男,但佳明,涂蕊,肖启程,朱子琦,唐丽..腹泻犬源大肠埃希菌耐药性以及整合子–基因盒检测研究[J].华南农业大学学报,2018,39(3):6-12,7.基金项目
国家自然科学基金(31000548) （31000548）
国家重点研发计划(2016YFD0501009) （2016YFD0501009）
成都大熊猫繁育研究基金(CPF2015-4) （CPF2015-4）
四川农业大学本科生科研兴趣培养项目(ky2016076) （ky2016076）
