华南农业大学学报2018，Vol.39Issue(3)：13-18,6.DOI:10.7671/j.issn.1001-411X.2018.03.003
广东地区口蹄疫病毒VP1和3A基因的序列分析
Sequence analysis of VP1 and 3A genes of foot-and-mouth disease virus in Guangdong area
摘要
Abstract
[Objective]To analyze the genetic variation of foot-and-mouth disease virus (FMDV) in Guangdong area, and provide fundamental data for studying FMDV infections as well as prevention and control of foot-and-mouth disease.[Method]We amplified and sequenced VP1 and 3A genes of FMDV from pathological samples of pigs in Guangdong area from 2015 to 2016, and analyzed the gene sequences.[Result]Eleven strains of type O FMDV strains belonged to the social spectrum type strains. Seven type A FMDV strains belonged to the Asia type strains.Comparing the VP1 genes of the 11 type O FMDV strains with pandemic strains, GD-MM-3 and O/BY/CHA/2010 strains had the highest sequence similarity being 95.3%, while strain GD-1, GD-MM-1 and GD-MM-3 had the highest sequence similarity of 90.8% compared to strain O/MYA/1/98.Comparing the VP1 genes of 7 type A FMDV strains with pandemic strains,A/GDMM/2013 and GD-CH-3 strains had the highest sequence similarity being 99.8%, while A/HuBWH/2009 and GD-3 strains had the highest sequence similarity being 91.4%. Amino acid deletion was not found in 3A proteins of 11 type O FMDV strains. The 3A proteins of 7 type A FMDV strains had multiple amino acid deletions.[Conclusion]The genetypes of FMDV in part area of Guangdong are complex, which leads to the difficulties of disease prevention and control.关键词
口蹄疫病毒/VP1基因/3A基因/序列相似性/遗传变异Key words
foot-and-mouth disease virus/VP1 gene/3A gene/sequence similarity/genetic variation分类
农业科技引用本文复制引用
李琪,邢秀琳,张桂红,谢增胜,石庆伟,陈耀,孙彦阔,冯松林,汪志,冀池海,刘艺兴..广东地区口蹄疫病毒VP1和3A基因的序列分析[J].华南农业大学学报,2018,39(3):13-18,6.基金项目
国家重点研发计划(2016YFD0500707) （2016YFD0500707）
