华南农业大学学报2018,Vol.39Issue(3):19-24,6.DOI:10.7671/j.issn.1001-411X.2018.03.004
2014—2016年广东省规模化猪场猪繁殖与呼吸综合征的流行病学调查
Epidemiological survey of porcine reproductive and respiratory syndrome in scaled pig farms of Guangdong province
摘要
Abstract
[Objective]To investigate the prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) in Guangdong province.[Method]From 2014 to 2016, totally 1 137 porcine tissue samples and 9 432 porcine serum samples were collected from scaled pig farms in Guangdong for antigen and antibody detection respectively. The results were compared among different years, seasons and regions. Phylogenetic analysis were performed on isolated viruses.[Result]From 2014 to 2016, the yearly positive rates of PRRSV antigens were 30.86%, 34.35% and 37.50% respectively, while the yearly antibody positive rates were 82.86%, 68.87% and 74.56% respectively. In quarterly analysis, the first quarter had the highest antigen positive rate of 40.27% and the second quarter had the lowest antigen positive rate of 28.66%. The antibody level of every quarter was above 70%. There were significant differences in PRRSV infection rates among different regions.The infection rates in eastern Guangdong and western Guangdong were significantly lower than those in the Pearl River Delta and northern Guangdong. The antibody positive rate was the lowest (69.51%) in eastern Guangdong and above 75% in the other three regions. A total of 69 PRRSV strains were isolated from samples with positive antigen, of which 39 strains belonged to Subgenotype I.[Conclusion]The antigen positive rate of PRRSV in Guangdong shows an increasing trend, while the antibody positive rate is relatively high but unstable. Subgenotype I is the predominant subgroup in Guangdong based on the virus isolation results.关键词
猪繁殖与呼吸综合征(PRRS)/流行病学调查/抗原检测/抗体检测/广东地区Key words
Porcine reproductive and respiratory syndrome (PRRS)/epidemiological investigation/antigen detection/antibody detection/Guangdong province分类
农业科技引用本文复制引用
冯松林,邢秀琳,陈耀,汪志,张桂红..2014—2016年广东省规模化猪场猪繁殖与呼吸综合征的流行病学调查[J].华南农业大学学报,2018,39(3):19-24,6.基金项目
国家生猪现代农业产业技术体系项目(CARS-36) (CARS-36)
公益性行业(农业)科研专项(201203039) (农业)
广东省高等学校优秀青年教师培养计划(YQ201530) (YQ201530)