华南农业大学学报2018，Vol.39Issue(3)：25-30,6.DOI:10.7671/j.issn.1001-411X.2018.03.005
1个水稻紫叶基因pl41的遗传分析与定位
Genetic analysis and mapping of a rice purple leaf gene pl41
摘要
Abstract
[Objective]To carry out genetic analysis and gene mapping of purple leaf character in the rice material pl41,and provide a foundation for pl41 gene map-based cloning,function research and application in rice breeding.[Method]The genetically stable purple leaf germplasm pl41 was derived from the hybrids of a purple leaf indica cultivar 'Z3474' and a green leaf japonica cultivar 'Nipponbare'. The phenotypic characteristics of pl41 were surveyed. pl41 was crossed with 'Nipponbare', then F1and F2segregation progenies were used for genetic analysis and preliminary mapping of pl41 gene.[Result]The purple character of pl41 firstly appeared in leaf sheath, leaf apex and leaf margin at seedling stage, then the aboveground parts of pl41 plant presented dark purple at heading stage. Compared to 'Nipponbare', chlorophyll content of pl41 was significantly higher at seedling stage, significantly lower at heading stage and there was no significant difference at filling stage. Anthocyanin contents of pl41 were all significantly higher than those of 'Nipponbare' at seedling, heading and filling stages. The purple leaf character of pl41 was controlled by a pair of recessive nuclear genes, which was mapped to a 289 kb region on the short arm of chromosome 12. By sequencing for coding sequences of five genes within this mapped region, no difference was found in coding sequences of these genes between Pl41 and 'Nipponbare'.[Conclusion]The region of rice purple leaf gene pl41 is mapped preliminarily.The exact determination of pl41 depends on fine mapping,sequence alignment analysis and transcription analysis of candidate genes.关键词
水稻/紫叶性状/遗传分析/基因定位/pl41基因Key words
rice(Oryza sativa L.)/purple leaf trait/genetic analysis/gene mapping/pl41 gene分类
农业科技引用本文复制引用
刘立龙,李允振,谢旭阳,徐一博,李容柏,覃宝祥..1个水稻紫叶基因pl41的遗传分析与定位[J].华南农业大学学报,2018,39(3):25-30,6.基金项目
亚热带农业生物资源保护与利用国家重点实验室开放课题(SKLCUSA-b201607) （SKLCUSA-b201607）
广西研究生教育创新计划项目(YCSW2017043) （YCSW2017043）
广西大学博士启动项目(XBZ160070) （XBZ160070）
