华南农业大学学报2018，Vol.39Issue(3)：31-38,8.DOI:10.7671/j.issn.1001-411X.2018.03.006
同源四倍体黑皮冬瓜的诱导与倍性鉴定
Induction and ploidy identification of autotetraploid black wax gourd
摘要
Abstract
[Objective]To explore efficient induction method of autotetraploid Benincasa hispida and seek a simple, convenient, large flux and accurate method of ploidy level identification.[Method]The seedlings of diploid B.hispida inbred lines were used as materials.Autotetraploid B.hispida was induced using different concentrations of colchicine and oryzalin at different times including 06:00 to 07:00, 12:00 to 13:00 and 17:00 to 18:00 to treat the growth points of seedlings. Ploidy levels of mutated plants were determined by using the methods based on plant morphology, cytology, flow cytometric analysis and chromosome numbers of root tips.[Result]There were certain induction effects by using different concentrations of colchicine and oryzalin at different times, but the induction rate of colchicine was overall higher than that of oryzalin, and the highest induction rate was 32.18% using 2 g·L–1colchicine from 06:00 to 07:00. The ploidy identification result from the combination of methods based on plant morphology, cytology and flow cytometric analysis was the same as the result based on chromosome numbers of root tips.[Conclusion]The optimum method of obtaining autotetraploid was using 2 g·L–1colchicine from 06:00 to 07:00. The combination of plant morphology, cytology and flow cytometric analysis was a simple, convenient, large flux and accurate way to identify ploidy level.关键词
冬瓜/同源四倍体/诱变/倍性鉴定/流式细胞术Key words
Benincasa hispida/autotetraploid/induction/ploidy identification/flow cytometric analysis分类
农业科技引用本文复制引用
万正林,周艳霞,武鹏,邓俭英,李立志,龙明华..同源四倍体黑皮冬瓜的诱导与倍性鉴定[J].华南农业大学学报,2018,39(3):31-38,8.基金项目
广西科学研究与技术开发项目(桂科攻1222009-3D) （桂科攻1222009-3D）
广西科技基地和人才专项(桂科AD17129042) （桂科AD17129042）
国家现代农业产业技术体系广西大宗蔬菜创新团队专项(nycytxgxcxtd-10-03) （nycytxgxcxtd-10-03）
广西农业科学院科技发展基金项目(桂农科2012JZ12) （桂农科2012JZ12）
