华南农业大学学报2018，Vol.39Issue(3)：39-47,9.DOI:10.7671/j.issn.1001-411X.2018.03.007
人参叶片中人参皂苷含量、关键酶基因表达和生态因子的相关性分析
Correlation analyses of ecological factors, ginsenoside contents and expression of associated key enzymes in Panax ginseng leaves
摘要
Abstract
[Objective]To clarify the physiological and ecological mechanisms of ginsenoside biosynthesis, and reveal the relationship between ecdogical/genetic factors and quality of medicinal materials.[Method]Cultivated four-year-old Panax ginseng leaves at different growth times were used as materials.The contents of eight ginsenosides (Rg1、Re、Rf、Rb1、Rb2、Rb3、Rc、Rd) in leaves were detected by HPLC. The expression of seven key enzyme genes (HMGR, FPS, SS, SE, DS, β-AS, CYP716A47) involved in ginsenoside biosynthesis in ginseng leaves were determined by real-time quantitative fluorescent PCR. The effect of ecological factors and the expression of key enzyme genes on the synthesis and accumulation of ginsenosides in P.ginseng leaves were investigated by correlation and grey relational analysis.[Result]The expression of key enzyme genes involved in ginsenoside synthesis was active from July 13th to September 29th, and the expression of different genes synergistically increased or decreased. The contents of ginsenoside Re and Rg1in P.ginseng leaves were the highest,reached the maximum of 60.30 and 39.38 mg·g–1respectively in the growth period of ginseng root from August 31st to September 13th. Temperature, photosynthetic active radiation (PAR), soil water potential and relative humidity all had significant correlation with ginsenoside contents in P. ginseng leaves(P<0.05).HMGR expression in P.ginseng leaves was negatively correlated with Rb2content (P<0.05),SS expression was negatively correlated with Rg1or Re content(P<0.05),and β-AS expression was negatively correlated with Rc content (P<0.05) and Rb2content (P<0.01). Grey correlation analysis results showed that the major ecological factors that influenced ginsenoside contents in P.ginseng leaves were temperature, PAR, soil water potential and relative humidity, with their grey correlation values ranging from 0.727 9 to 0.871 1.[Conclusion]Under the regulation and control of ecological factors, the synthesis and accumulation of ginsenosides were influenced by the expression of involved key enzyme genes.关键词
人参/生态因子/人参皂苷/基因表达/相关性/灰色关联度Key words
Panax ginseng/ecological factor/ginsenoside/gene expression/correlation/grey correlation degree分类
农业科技引用本文复制引用
杨林林,张涛,杨利民,韩梅..人参叶片中人参皂苷含量、关键酶基因表达和生态因子的相关性分析[J].华南农业大学学报,2018,39(3):39-47,9.基金项目
国家中药材产业技术体系(CARS-21) （CARS-21）
国家自然科学基金(31270371) （31270371）
吉林省重大科技成果转化项目(20170307009YY) （20170307009YY）
