华南农业大学学报2018，Vol.39Issue(3)：54-59,6.DOI:10.7671/j.issn.1001-411X.2018.03.009
枯草芽孢杆菌BSF01菌剂制备及对高效氯氰菊酯的降解效果
Preparation of bacterial agent based on Bacillus subtilis BSF01 and its biodegradation effect on beta-cypermethrin
摘要
Abstract
[Objective]To prepare a bacterial agent that is able to degrade beta-cypermethrin (β-CP).[Method]Based on previously isolated and cultured Bacillus subtilis BSF01 which had excellent potential in rapidly degrading β-CP, the bacterial formulation was determined by optimizing additives and dosage. The degrading ability of prepared bacterial agent was evaluated in β-CP-contaminated soil under laboratory conditions.[Result]The optimal formulation was determined to blend 30% bacterial powder with 5% sodium dodecyl benzene sulfonate as wetting agent, 7% acacia gum as dispersant and 4% potassium phosphate as stabilizer, appending kaolin as the carrier to 100%. The quality assessment verified that the spore intensity of prepared bacterial agent reached 6.50×1011cfu·g–1with low water content (≤0.6%), high fineness (≥99%), short wetting time (32 s) and 78% suspensibility. The prepared agent was sprayed on β-CP-contaminated soil by diluting to 100, 1 000 and 2 000 times, respectively. After five days, about 87.50% of residual β-CP in soil was degraded by bacterial agent at 100-fold dilution, which rapidly decreased the residual level of β-CP in soil.[Conclusion]The prepared β-CP bacterial agent exhibits outstanding potential in realistic application with further optimization, which may provide an effective, safe and promising bacterial product for eliminating pyrethroid contamination in agricultural environment.关键词
高效氯氰菊酯/枯草芽孢杆菌/生物降解/菌剂/农药Key words
beta-cypermethrin/Bacillus subtilis/biodegradation/bacterial agent/pestiside分类
农业科技引用本文复制引用
周亮成,李运,李卓苗,林培炯,钟国华..枯草芽孢杆菌BSF01菌剂制备及对高效氯氰菊酯的降解效果[J].华南农业大学学报,2018,39(3):54-59,6.基金项目
广东省科技计划项目(2015B020207003) （2015B020207003）
广东大学生科技创新培育专项(PDJH2016B0085) （PDJH2016B0085）
