湖南中医药大学学报2018，Vol.38Issue(5)：493-498,6.DOI:10.3969/j.issn.1674-070X.2018.05.002
黄连素联合顺铂诱导人肺癌耐药A549/DDP细胞凋亡的实验研究
An Experimental Study on Apoptosis of A549 / DDP Cells Induced by Berberine Combined with Cisplatin
摘要
Abstract
Objective To observe the effect of berberine combined with cisplatin on cell apoptosis of A549 / DDP and investgate its related mechanism. Methods Human lung cancer resistance A549 / DDP cells, as the object of study, were added with 12 μg/mL cisplatin, and 20 μmol/L, 40 μmol/L, 80 μmol/L berberine combined with 12 μg/mL cisplatin, respectively, blank control wells (PBS wells) were set up, they were all treated with drugs for 24 h, 48 h and 72 h. The cell proliferation was evaluated by MTT assay. After intervention with drugs for 48 h, cell apoptosis was determined by using the flow cytometry instrument. The protein and mRNA levels of Caspase-3, Caspase-9, Bax, Bcl-2 were measured by Real-time PCR and western blotting analysis, respectively. Results Berberine combined with cisplatin could inhibit the growth of A549 /DDP cells (P<0.01) and promote apoptosis of A549/DDP cells (P<0.01), with a certain time-concentration dependence. Different concentrations of berberine combined with cisplatin increased the levels of protein and mRNA levels of apoptosis genes Caspase-3, Caspase-9, Bax (P<0.05), and down-regulated the protein and mRNA levels of anti-apoptosis gene Bcl-2 (P<0.05).Conclusion The molecular mechanism of berberine combined with cisplatin in inhibiting proliferation and promoting apoptosis of A549 / DDP cells may be related to up-regulating the expression of apoptosis related proteins Caspase-3, Caspase-9 and Bax, downregulating the expression of anti-apoptosis protein Bcl-2.关键词
黄连素/顺铂/肺癌耐药/细胞凋亡Key words
berberine/cisplatin/lung cancer drug resistance/cell apoptosis分类
医药卫生引用本文复制引用
曾玲芳,王亿君,吴依辰,章慧..黄连素联合顺铂诱导人肺癌耐药A549/DDP细胞凋亡的实验研究[J].湖南中医药大学学报,2018,38(5):493-498,6.基金项目
湖南省科学技术厅资助项目(2014FJ3133). （2014FJ3133）
