湖南中医药大学学报2018，Vol.38Issue(5)：499-503,5.DOI:10.3969/j.issn.1674-070X.2018.05.003
蛴螬不同途径给药对干性年龄相关性黄斑变性模型Caspase-3、FasL、TNF-α、NF-κB表达的影响
Effect of Different Drug Administration Approaches of Grub on the Expression of Caspase-3, FasL, TNF-α and NF-κB of Age-Related Macular Degeneration Models
摘要
Abstract
Objective To study the interventional mechanism of oral administration and eye drops of the grub extract on the age -related macular degeneration (AMD) model. Methods A Dry AMD living model was established by light damage method. Models were given grubs extract oral administration and eye drops respectively. After 4 weeks, the expression of cysreine aspastic acid-specific protease 3 (Caspase-3), nuclear factor-κB (NF-κB), Fas ligand (FasL), tumor necrosis factor-α (TNF-α) was observed. Results Compared with the blank group, the expression of Caspase-3 in the retina tissue of model group decreased significantly, and the expression of FasL, TNF-α and NF- κB increased (P<0.05). Compared with the blank group, all indexes of the oral and eye drops groups had no significant difference (P>0.05). Compared with the model group, the expression of Caspase-3 increased significantly in the oral group and the eye drops group, and the expression of FasL, TNF -α and NF -κB decreased significantly. Compared with the oral group, there was no significant difference in all indicators in the eye drops group (P>0.05). Conclusion Grub extract has regulating action on photoreceptor cells apoptosis, such as performance for early suppression and lately acceleration. Its regulating effect is probably achieved by inhibiting or promoting the expression of Caspase-3. Grub extract shows inhibiting effect on chronic inflammation by inhibiting the expression of related inflammation factors, such as FasL, TNF-α, NF-κB.关键词
蛴螬提取物/给药途径/年龄相关性黄斑变性/半胱氨酸天冬氨酸蛋白酶-3/肿瘤坏死因子α/跨膜蛋白/核转录因子κBKey words
grub extract/administration route/age-related macular degeneration/cysreine aspastic acid-specific protease-3/tumor necrosis factor-α/fas ligand/nuclear factor-κB分类
医药卫生引用本文复制引用
谭涵宇,李建超,彭俊,文小娟,周亚莎,彭清华..蛴螬不同途径给药对干性年龄相关性黄斑变性模型Caspase-3、FasL、TNF-α、NF-κB表达的影响[J].湖南中医药大学学报,2018,38(5):499-503,5.基金项目
湖南省中医药科研重点项目(201438) （201438）
中医药防治眼耳鼻咽喉疾病湖南省重点实验室建设项目(2017TP1018) （2017TP1018）
湖南省研究生创新课题(CX2014B352) （CX2014B352）
长沙市科技计划项目(KC1704005) （KC1704005）
湖南省中医五官科学重点学科建设项目 （）
中央财政支持地方高校资助项目. （）
