湖南中医药大学学报2018，Vol.38Issue(5)：504-507,4.DOI:10.3969/j.issn.1674-070X.2018.05.004
心痛方对急性心肌梗死大鼠心肌组织内游离钙离子浓度及RYR2、PLB mRNA表达的影响
Effect of Xintong Fang on the Free Calcium Concentration and Expression of RYR2 and PLB mRNA in Cardiac Muscular Tissue of Rats with Acute Myocardial Infarction
摘要
Abstract
Objective To observe the effect of Xintong Fang on intracellular free calci um concentration (Ca2+), and RYR2 and PLB mRNA expression in cardiomyocytes of acute myocardial infarction (AMI) rats, and to explore the significance of calcium homeostasis in AMI and the therapeutic mechanism of Xintong Fang. Methods Fifty SD rats were divided into the sham operation group, model group, high dose of Xintong Fang group, low dose of Xintong Fang group, diltiazem group. The AMI model was made by improved coronary ligation, and the Ca2 +in myocardium was detected by laser scanning copolymerization microscope, and the infarct size was measured by HE staining, and the expression of RYR2 and PLB mRNA in myocardium was measured by RT-PCR. Results Compared with the sham operation group, Ca2+in the drug intervention groups and model group increased, the expression of RYR2 and PLB mRNA decreased, the difference was statistically significant (P<0.01). Compared with the model group, myocardial infarction area and Ca2+in each drug intervention groups decreased, the expression of RYR2 and PLB mRNA expression increased (P<0.01). Compared with the diltiazem group, the myocardial infarction area and Ca2+in Xintong Fang groups reduced, RYR2 and PLB mRNA expression increased (P<0.01 or P<0.05). Compared with the low-dose of Xintong Fang group, the myocardial infarction area and Ca2+in the high-dose of Xintong Fang group reduced, the expression of PYR2 and PLB mRNA increased (P<0.01 or P<0.05). Conclusion Xintong Fang can reduce myocardial infarction area in rats with acute myocardial infarction, and has more advantages than sulfiazem group. Xintong Fang could decrease the concentration of Ca2+, increase the expression of RYR2 and PLB mRNA, and regulate calcium homeostasis. Its protective effect on AMI rats may be related to reduced myocardial damage of calcium overload and enhanced.关键词
急性心肌梗死/心痛方/Ca2+/RYR2/PLBmRNA/钙稳态Key words
acute myocardial infarction/Xintong Fang/Ca2+/RYR2/PLB mRNA/calcium homeostasis分类
医药卫生引用本文复制引用
刘志云,范金茹,周斐然,陈彤,王建湘,欧阳过,廖建萍,谢荣苑..心痛方对急性心肌梗死大鼠心肌组织内游离钙离子浓度及RYR2、PLB mRNA表达的影响[J].湖南中医药大学学报,2018,38(5):504-507,4.基金项目
湖南省教育厅科学研究项目(16A159). （16A159）
