湖南中医药大学学报2018，Vol.38Issue(5)：508-512,5.DOI:10.3969/j.issn.1674-070X.2018.05.005
黄桷兰乙醇溶液对小鼠止痒作用的初步研究
Preliminary Study of Antipruritic Effect of Huangjuelan Ethanol Solution on Mice
摘要
Abstract
Objective To explore the antipruritic effect of Huangjuelan ethanol solution on the mosquito biting model mice. Methods 60 mice, half male and half female, were selected and 10 mice in each group. Itching model was established through the intradermal injection of histamine phosphate at the back of mouse ear. Different concentrations of Huangjuelan ethanol solution and other treatment were applied on the mouse ear, scratching frequency and scratching latency were observed and recorded within 30 minutes at the wake of intradermal injection of histamine phosphate. All the mice were sacrificed by cutting heads, blood was collected. Serum concentration of IL-6 was detected with ELISA. Skin tissues of mice ear were collected to prepare for the pathological section, inflammatory cell infiltration as well as hyperplasia degree was observed under microscope. SPSS 20.0 was used for statistical analysis. Results The low concentration of Huangjuelan ethanol solution (0.02 g/mL) reduced the scratching frequency significantly (P<0.01), while high concentration of Huangjuelan ethanol solution(0.5 g/mL) could significantly inhibit the inflammatory cell infiltration and hyperplasia of mouse ear. No significant difference in serum IL-6 concentration was observed among these groups (P>0.05). Conclusion Huangjuelan ethanol solution has a certain inhibitory effect on itching in mice, and low concentration of Huangjuelan solution shows better inhibitory effect. Therefore, it is still necessary to increase the sample size in future studies, and then to explore the mechanism of action of ethanol solution of this plant.关键词
瘙痒/黄桷兰/组胺/IL-6Key words
itching/Huangjuelan ethanol solution/histamine/IL-6分类
医药卫生引用本文复制引用
张锐,凌瑜双,梁和,熊强,刁华,汪洋..黄桷兰乙醇溶液对小鼠止痒作用的初步研究[J].湖南中医药大学学报,2018,38(5):508-512,5.基金项目
重庆医科大学大学生科研与创新实验项目(201420). （201420）
