湖南中医药大学学报2018，Vol.38Issue(5)：541-545,5.DOI:10.3969/j.issn.1674-070X.2018.05.014
艾灸对荷瘤胃癌大鼠外周血中CD3-CD161+NK与CD3+CD161+NKT含量的影响
Effect of Moxibustion on CD3-CD161+NK and CD3+CD161+NKT in Peripheral Blood of Tumor Bearing Gastric Carcinoma Rats
摘要
Abstract
Objective To discuss the effect of moxibustion on CD3-CD161+NK and CD3+CD161+NKT in peripheral blood of tumor bearing gastric carcinoma rats. Methods 50 healthy SD rats were randomly divided into 5 groups: blank group, control group, model group, moxibustion group and infrared group, 10 rats in each group, half male and half female. Walker-256 cells were used to prepare gastric carcinoma model by situ implantation of tumor tissue. Moxibustion group was given suspended moxibustion at zhongwan (CV12), Zusanli (ST36). The infrared group was given short wave infrared radiation for 20 minutes on the stomach and lower back, once daily. The changes of behavior were observed, and body mass, diet and drinking water were recorded. At the 22nd day, after fasting for 12 hours, orbital blood of rats were collected. The changes of CD3-CD161+NK and CD3+CD161+NKT in peripheral blood were determined by flow cytometry instrument. Results Compared with blank group, the body mass and eating capacity in model group decreased significantly (P<0.05), and CD3+CD161+NKT in peripheral blood increased (P<0.05). Compared with model group, quality and eating capacity of rats in later period treatment improved in moxibustion group obviously (P<0.05), and CD3-CD161+NK, CD3+CD161+NKT in peripheral blood increased (P<0.05). Compared with infrared group, the improvement of body mass and eating capacity was more obvious in moxibustion group, and CD3-CD161+NK, CD3+CD161+NKT increased in peripheral blood (P<0.05). Conclusion Moxibustion could increase body mass, recover eating capacity, and improve state of SD rats with gastric carcinoma. The mechanism may be related to the increase of CD3-CD161+NK and CD3+CD161+NKT in peripheral blood and enhancement of NK cell immunization.关键词
胃癌/艾灸/模型大鼠/CD3-CD161+NK/CD3+CD161+NKTKey words
gastric carcinoma/moxibustion/model rats/CD3-CD161+NK/CD3+CD161+NKT分类
医药卫生引用本文复制引用
欧阳里知,谭静,赵欢,彭卓隽,戴丽雯,陈欲攀,李铁浪,林亚平..艾灸对荷瘤胃癌大鼠外周血中CD3-CD161+NK与CD3+CD161+NKT含量的影响[J].湖南中医药大学学报,2018,38(5):541-545,5.基金项目
国家自然科学基金面上项目(81574077). （81574077）
