基础医学与临床2018，Vol.38Issue(5)：626-631,6.
β1-肾上腺素受体自身抗体通过促进肝细胞凋亡参与小鼠肝损伤
Hepatocytes apoptosis induced by β1-adrenoceptor autoantibody is involved in hepatic dysfunction
摘要
Abstract
Objective To investigate the effect of β1-Adrenoceptor autoantibody on liver function.Methods The biologically active of β1-AA was prepared and passive immunization model was established with β1-AA.The bio-chemical parameters of the liver were measured by the automatic serum biochemical analyzer.The liver size, hepatic vein,portal vein velocity were detected by liver ultrasound;hepatocytes apoptosis were tested by tunel stai-ning,annexin V/PI staining and caspase 3 activity detection.Results The biologically active of β1-AA and passive immunization model were established successfully.The ALT and AST of the liver significantly increased and the ALB decreased in the passive immunization process.The apoptosis of the hepatocytes increased,and meto-prolol partially reversed this effect.Conclusions β1-AA may induce hepatocytes apoptosis by β1-adrenergic receptor and participate in the development of liver injury.关键词
β1-肾上腺素受体/自身抗体/肝细胞/凋亡Key words
β1-adrenergic receptor/autoantibody/hepatic cells/apoptosis分类
医药卫生引用本文复制引用
于海存,杜芸辉,张诗晗,闫莉,李玉明..β1-肾上腺素受体自身抗体通过促进肝细胞凋亡参与小鼠肝损伤[J].基础医学与临床,2018,38(5):626-631,6.基金项目
国家自然科学基金(81271165,81470540) （81271165,81470540）
北京市自然科学基金(7142019) （7142019）
