基础医学与临床2018，Vol.38Issue(5)：632-637,6.
160例非人源细胞系的种间交叉污染情况分析
Analysis of inter-species cross-contamination in 160 non-human cell lines
摘要
Abstract
Objective To analyze interspecies cross-contamination of 160 non-human cell lines.Methods One hundred and sixty common non-human cell lines were collected and their species were identified by PCR.For the suspicious cells,chromosome analysis was further used to confirm their species.Results Six in 160 non-human cell lines were cross-contaminated.A rat cell line was mixed by a human cell line,and 5 were totally cross-con-taminated,and were indentified as wrong species.Conclusions Species identification is an indispensable part of cell quality control.Each cell line should undergo a full QA(Quality Assurance)assessment before it is used for research.关键词
非人源细胞系/细胞培养/质量控制/种属鉴定Key words
non-human cell lines/cell culture/quality control/species identification分类
医药卫生引用本文复制引用
卞晓翠,杨振丽,左春霞,周芳颖,刘玉琴..160例非人源细胞系的种间交叉污染情况分析[J].基础医学与临床,2018,38(5):632-637,6.基金项目
国家科技基础条件平台(NSTI-CR17) （NSTI-CR17）
