解放军医学杂志2018，Vol.43Issue(3)：206-210,5.DOI:10.11855/j.issn.0577-7402.2018.03.05
慢性间歇性缺氧对大鼠肝脏糖代谢的影响及机制
Effect of chronic intermittent hypoxia on glycometabolism in rat' liver and the mechanism thereof
摘要
Abstract
Objective To investigate the effects of chronic intermittent hypoxia on the adipose factor and the expressions of insulin receptor substrate 2 (IRS-2),glucose transporter 2 (GLUT-2) and leptin in rat liver.Methods Twenty-four mature SD rats were randomly divided into 3 groups:control group (UC),chronic intermittent hypoxia group (CIH) and reoxygenation group (RH).The arterial blood gas analysis was performed after the establishment of rat model.The serum fasting blood glucose (FBG) and fasting insulin (FINS) in each group were detected by peroxidase method;the concentrations of free fatty acids (FFA) and leptin were detected by ELISA.The expressions ofmRNA and protein of GLUT-2,IRS-2 and leptin were detected by qRT-PCR and Western blotting.Results The serous concentrations of FBG,FINS,FFA and leptin were significantly higher in CIH group than in UC group (P＜0.05),and were dramatically higher in RH group than in both CIH group (P=0.003) and UC group (P=0.000).Western blotting and qRT-PCR detection showed that the protein and mRNA expressions of GLUT-2 and IRS-2 were significantly lower in CIH group than in RH group of rat liver (P＜0.05),while were markedly lower in RH group than in UC group (P＜0.05);the expressions of leptin protein and mRNA were significantly higher in CIH group than in RH group (P＜0.05),while were obviously higher in RH group than in UC group of rat liver (P＜0.05).Conclusion Insulin resistance induced by chronic intermittent hypoxia may be associated with the elevation of serum FFA and leptin,and be related to the decreased expression of GLUT-2 and IRS-2 and increased expression of leptin in liver.关键词
慢性间歇性缺氧/胰岛素抵抗/葡萄糖转运蛋白2/胰岛素受体底物2/瘦素/游离脂肪酸Key words
chronic intermittent hypoxia/insulin resistance/glucose transporter 2/insulin receptor substrate 2/leptin/free fatty acid分类
医药卫生引用本文复制引用
余维,覃泽平,陈华娇,张峰,李兵..慢性间歇性缺氧对大鼠肝脏糖代谢的影响及机制[J].解放军医学杂志,2018,43(3):206-210,5.基金项目
This work was supported by the Key Research Project of Chongqing Municipal Bureau of Health (2012-1-059)重庆市卫生局重点科研项目(2012-1-059) （2012-1-059）
