解放军医学杂志2018，Vol.43Issue(3)：211-216,6.DOI:10.11855/j.issn.0577-7402.2018.03.06
Hsa_circ_PVT1在肝细胞癌中的表达及意义
Expression of hsa_circ_PVT1 in human hepatocellular carcinoma and its clinical significance
朱元鑫 1付航玮 1林夏 1蓝翔 1马钰 1李光耀 1董睿 1王宇洲 1陈平1
作者信息
- 1. 400042 重庆 陆军军医大学大坪医院野战外科研究所肝胆外科
- 折叠
摘要
Abstract
Objective To determine the expression and clinical significance of circ-PVT1 in human hepatocellular carcinoma (HCC) and its effect on HCC cell proliferation.Methods The expressions of circ-PVT1 in hepatocellular carcinoma and the matched tumor-adjacent tissues were detected by RT-qPCR and the relationship between pathological indexes and the expression level was analyzed in 46 patients.The expressions of circ-PVT1 in human normal liver cell line (L02) and hepatocellular carcinoma cell lines (HepG2,SMMC-7721,MHCC-97H,MHCC-97L,HCC-LM3) were detected by RT-qPCR and were compared thereafter.With knocking down the expression of circ-PVT1,si-circPVT1 was transfected into HepG2 and SMMC-7721 cells by using lipofectamine technique in vitro,with the si-NC being taken as negative control.After interfering the expression of circ-PVT1,the effect on the proliferation of hepatocellular carcinoma cells was detected by CCK-8 and EDU experiments and flow cytometry was conducted to observe the effect of circ-PVT1 on cell cycle.Results The expression level of circ-PVT1 was significantly higher in HCC tissues than in adjacent tissues (P＜0.01),and its high expression level was significantly correlated with tumor size,TNM stage and differentiation degree.Similarly,in human hepatocellular carcinoma cell lines (HepG2,SMMC-7721,MHCC-97H,MHCC-97L,HCC-LM3),the expression level of circ-PVT1 was also higher than that in human normal liver cell line L02 (P＜0.05).Compared with the negative control group,silencing of circ-PVT1 resulted in remarkable reduction in cell proliferation of HepG2 and SMMC-7721.Conclusion circ-PVT1 may act as a potential biomarker for HCC diagnosis and may become a novel proliferation factor.关键词
肝细胞癌/环状RNA/PVT1/细胞增殖/细胞周期Key words
hepatocellular carcinoma/circular RNA/PVT1/cell proliferation/cell cycle分类
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朱元鑫,付航玮,林夏,蓝翔,马钰,李光耀,董睿,王宇洲,陈平..Hsa_circ_PVT1在肝细胞癌中的表达及意义[J].解放军医学杂志,2018,43(3):211-216,6.
