集美大学学报（自然科学版）2018，Vol.23Issue(2)：81-90,10.
黄姑鱼(Nibea albiflora)gsdf基因的克隆及表达分析
Cloning and Expression Analysis of gsdf Gene in the Yellow Drum(Nibea albiflora)
摘要
Abstract
In order to study the function of gsdf (gonadal soma derived factor) in sex differentiation and gonad development in the yellow drum(Nibea albiflora ,the ORF (open reading frame) sequence of gsdf gene was obtained through molecular and bioinformatics technique.And the expression profile of gsdf in differ-ent tissues and different development stages of gonads was assayed by qRT -PCR (quantitative real time PCR).Results showed that the gsdf contained a 648 bp open reading frame encoding a protein of 215 amino acids with a conserved domain shared by the TGF-β superfamily.The qRT-PCR analysis revealed that gsdf mainly expressed in testis of the yellow drum.Hatching and rearing at 23 ℃,the gsdf expression in the genetic males was detected from 40 dph (day post hatch) while the testis was unidentifiable by histology.The expres-sion of gsdf went up rapidly after 49 dph,and reached to the maximum peak on 120 dph when the testis was fully differentiated.Subsequently,the expression of gsdf showed down-regulation and it tended to be stabilized after the testis was fully mature.These results indicated that gsdf was tightly related to the testis differentiation and development in N.albiflora.关键词
黄姑鱼/gsdf基因/组织表达/时序表达Key words
yellow drum(Nibea albiflora/gsdf (gonadal soma derived factor)/tissue expression pat-tern/temporal expression分类
生物科学引用本文复制引用
孙莎,韩兆方,李完波,叶坤,林爱强,崔晓莹,王志勇..黄姑鱼(Nibea albiflora)gsdf基因的克隆及表达分析[J].集美大学学报（自然科学版）,2018,23(2):81-90,10.基金项目
厦门市海洋经济发展专项资金项目(14GZY70NF34) （14GZY70NF34）
福建省农业高校产学合作重大项目(2014N5011) （2014N5011）
