解剖学杂志2018，Vol.41Issue(2)：147-151,5.DOI:10.3969/j.issn.1001-1633.2018.02.005
淫羊藿苷对环磷酰胺诱导生精障碍大鼠下丘脑-垂体-睾丸轴的影响
Effects of icariin on hypothalamus-pituitary-testicular axis of rat with impaired spermatogenesis induced by cyclophosphamide
摘要
Abstract
Objective:To explore the role of icariin in the animal models of the cyclophosphamide-induced rat testis spermatogenic failure,and study the effects of icariin on the hypothalamus-pituitary-testicular axis.Methods:Rats were divided into blank control group,negative control group,model group and icariin treatment group.H-E staining was used to observe the morphological structural changes in testicular tissue.TUNEL method was used to detect the apoptosis of testicular germ cells.Serum testosterone,follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels were detected by radioimmunoassay.GnRH nerve fibers in median eminence of the rat hypothalamus were detected by SABC immunohistochemistry.Results:H-E staining observation of testicular tissue in model group rats showed the testis seminiferous tubule diameter narrowed,the spacing between seminiferous tubules widened,and the seminiferous epithelium became thinner.The number of germ cells decreased,and sperm was rarely found in seminiferous tubules.Compared with blank control group,there was significant change in seminiferous tubules.Compared with model group,treatment group showed signs of thickening seminiferous tubule epithelium and significant increase of seminiferous tubules with sperms.Model group showed obvious increase of germ cell apoptosis in testicular seminiferous tubules compared with blank control group.Treatment group showed obvious decrease of germ cell apoptosis in seminiferous tubules compared with model group.Serum testosterone of the model group was significantly decreased compared with the blank control group;serum testosterone of treatment group was significantly increased compared with model group.There was no significant difference in serum FSH and LH level between these groups.GnRH-positive fibers in the median eminence of model group showed signs of increased GnRH-positive fibers,deeper staining and significant change in optical density compared with blank control group.Compared with model group,treatment group showed signs of reduced GnRH-positive fibers and lighter staining.There were no significant differences in all the parameters between the negative control group and the blank control group.Conclusion:Icariin improves the testicular seminiferous tubule structure,and promotes spermatogenesis and testosterone secretion of Leydig cells.Icariin reduces the apoptosis of the testicular germ cells and the content of GnRH in the median eminence.关键词
睾丸/下丘脑/淫羊藿苷/环磷酰胺/生精障碍/睾酮/大鼠Key words
testis/hypothalamus/icariin/cyclophosphamide/impaired spermatogenesis/testosterone/rat引用本文复制引用
高学勇,林珊,韩咪莎..淫羊藿苷对环磷酰胺诱导生精障碍大鼠下丘脑-垂体-睾丸轴的影响[J].解剖学杂志,2018,41(2):147-151,5.基金项目
福建省自然科学基金(2015J01676) （2015J01676）
