解剖学杂志2018，Vol.41Issue(2)：163-166,201,5.DOI:10.3969/j.issn.1001-1633.2018.02.009
微囊化嗅鞘细胞移植周围神经损伤痛及下调背根神经节P2X3嘌呤受体的表达
Microencapsulated olfactory ensheathing cells transplantation alleviates peripheral nerve injury pain and down-regulates P2X3 purine receptor expression in dorsal root ganglion
摘要
Abstract
Objective:To investigate the effect of microencapsulated and non-microencapsulated olfactory ensheathing cells (OECs) transplantation on peripheral nerve injury and the expression of P2X3 purine receptor.Methods:NASH method was used to cultivate and expand cells from olfactory bulb tissue of a SD rat.SD rats were randomly divided into sham group,model group,non-microencapsulater-OECs (non-MC-OECs group) and microencapsulated OECs group (MC-OECs groups).The 10th and 20th days after surgery,the mechanical stimulation constraining thresholds of the rats in each group were measured by behavioral methods.P2X3 receptor positive cells percentage and average absorbance were observed by in situ hybridization histochemistry.Results:The 10th and 20th days after surgery,the mechanical stimulation constraining thresholds of rats were increased from the model group,the non-MC-OECs group,the MC-OECs group to the sham group,and the positive cells percentage and average absorbance of P2X3 receptor were decreased in turn.Compared with CCI group,the mechanical stimulation constraining thresholds of rats were increased,and the positive cells percentage and average absorbance of P2X3 receptor were decreased in the non-MC-OECs group.Compared with non-MC-OECs group,the mechanical stimulation constraining thresholds of rats were increased,the positive cells percentage and average absorbance of P2X3 receptor were decreased in the MC-OECs group.The difference between groups was statistically significant.Conclusion:Microencapsulated OECs transplantation can better alleviate chronic peripheral nerve injury pain and reduce the expression level of P2X3 receptor than non-MC-OECs on the 20th day after surgery.关键词
周围神经损伤/嗅鞘细胞/微囊化/坐骨神经/背根神经节/P2X3嘌呤受体Key words
peripheral nerve injury/olfactory ensheathing cells/microencapsulation/sciatic nerve/dorsal root ganglion/P2X3 receptor引用本文复制引用
张文俊,熊梅娟,刘怡果,薛国勇,刘曾旭..微囊化嗅鞘细胞移植周围神经损伤痛及下调背根神经节P2X3嘌呤受体的表达[J].解剖学杂志,2018,41(2):163-166,201,5.基金项目
国家自然科学基金(81260190,81760418) （81260190,81760418）
江西省自然科学基金(20132BAB205023) （20132BAB205023）
江西省卫生计生委计划(20173010) （20173010）
