解剖学杂志2018，Vol.41Issue(2)：167-171,5.DOI:10.3969/j.issn.1001-1633.2018.02.010
血管活性肠肽抑制帕金森病MPTP模型小鼠纹状体中核因子-κB p65、肿瘤坏死因子α和单核细胞趋化蛋白-1的上调
Vasoactive intestinal peptide inhibits the upregulation of NF-κB p65, TNFα and MCP-1 in the MPTP mouse model of Parkinson's disease
摘要
Abstract
Objective:To investigate the effect of vasoactive intestinal peptide (VIP) on the neuroinflammation and the protein level of nuclear factor-κB p65 (NF-κB p65) in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson's disease (PD).Methods:Thirty male C57BL/6J mice were randomly divided into normal saline (NS) group,MPTP group and MPTP+VIP.Immunohistochemistry was used to observe the level of tyrosine hydroxylase (TH),glial cell fibrous acid protein (GFAP) and ion-calcium-binding protein (Iba-1).The contents of tumor necrosis factor alpha (TNFα) and monocyte chemotactic protein 1 (MCP-1) in the striatum were detected by ELISA.The level of NF-κB p65 was measured by Western blotting.Results:Compared with NS group,the level of TH was significantly decreased,the level of GFAP and Iba-1 was obviously elevated,and the contents of TNF-α,MCP-1 and the level of NF-κB p65 in the striatum of MPTP group were significantly increased.However,VIP sufficiently ameliorated TH depletion in the striatum of the MPTP-induced mice,and evidently suppressed the activation of microglia and astrogliosis.In addition,VIP inhibited the abnormal elevation of the content of TNFα and MCP-1,and restrained the level of NF-κB p65 in the striatum of MPTP-induced mice.Conclusion:VIP can effectively reverse the neurological damage of the striatum in the MPTP-induced mice by down-regulating the level of NF-κB p65.关键词
帕金森病/血管活性肠肽/核因子-κB p65/单核细胞趋化蛋白-1/神经炎症/小鼠Key words
Parkinson's disease/vasoactive intestinal peptide/nuclear factor-κB p65/monocyte chemotactic protein 1/neuroinflammation/mouse引用本文复制引用
吕娥,刘飞,李侃,李晓健,费学超,卢克良..血管活性肠肽抑制帕金森病MPTP模型小鼠纹状体中核因子-κB p65、肿瘤坏死因子α和单核细胞趋化蛋白-1的上调[J].解剖学杂志,2018,41(2):167-171,5.基金项目
山东省医药卫生科技发展计划项目(2015WS0063) （2015WS0063）
潍坊医学院大学生科技创新基金(KX2016011) （KX2016011）
潍坊医学院博士启动基金(2017BSQD26) （2017BSQD26）
