实用临床医药杂志2018，Vol.22Issue(9)：1-5,5.DOI:10.7619/jcmp.201809001
血管生成素样蛋白4对M2型巨噬细胞分化的影响
Effect of ANGPTL 4 on M2 macrophages differentiation
摘要
Abstract
Objective To analyze the effect of angiopoietin-like protein 4(ANGPTL4) on M2 macrophage differentiation.Methods The frequency of M2 macrophages in spleen of ANGPTL 4-/-mice and the controls was detected by flow cytometry.And the changes of M2 macrophages was measured by LPS stimulation.F4/80 + macrophages was separated by flow cytometry and treated with LPS or recombinant ANGPTL 4 protein.The secretion of IL-4 in CD4 + T cells was observed after purified macrophages after co-culture with CD4 + T cells by the flow-cytometric intracellular staining method.Results There was no significant difference in the frequency of M2 macrophages in ANGPTL 4-/-mice and controls.LPS stimulation did not affect the expression of M2 macrophages from ANGPTL 4-/-mice.The macrophages from ANGPTL 4/-mice did not promote differentiation of CD4 + T cells into Th2 cells.After co-culturing of macrophages and CD4 + T cells for 48 h in vitro,IL-4 secretion of CD4 + T cells was not changed.Conclusions ANGPTL4 has no effects on M2 macrophage differentiation.关键词
血管生成素样蛋白4/M2型巨噬细胞/肿瘤免疫逃逸/分化Key words
angiopoietin-like 4/M2 macrophage/tumor immune escape/differentiation分类
医药卫生引用本文复制引用
吴丹丹,丁时桢,路国涛,肖炜明,龚卫娟..血管生成素样蛋白4对M2型巨噬细胞分化的影响[J].实用临床医药杂志,2018,22(9):1-5,5.基金项目
国家自然科学基金(81671547,81471547) （81671547,81471547）
江苏省大学生创新创业训练计划(201611117093X) （201611117093X）
