军事医学2018，Vol.42Issue(1)：38-43,6.DOI:10.7644/j.issn.1674-9960.2018.01.010
ZNF331过表达对结肠癌细胞增殖与凋亡的影响
Effects of ZNF331 overexpression on human colon cancer cell proliferation and apoptosis
摘要
Abstract
Objective To investigate the effects of ZNF331 overexpression on proliferation and apoptosis of human colon cancer cell HCT116, and the relevant apoptotic mechanism.Methods The lentivirus vector of overexpressed ZNF331,Flag-pLV-Neo-ZNF331,was constructed and packaged.HCT116/p53 +/+(wild type p53)and HCT116/p53 -/-(deficient p53)cells were infected.Clones with ZNF331 overexpression were identified by Western blotting.Cell proliferation assay,colony formation assay and flow cytometry analysis were used to examine the effects of ZNF 331 on cell proliferation and apoptosis.Immunoprecipitation,luciferase reporter gene assay and real-time PCR were performed to detect interactions between ZNF331 and p53, p53 transcriptional activity and the expression of p 53 apoptotic target genes, respectively.Results The lentivirus vector of overexpressed ZNF 331 was successfully generated.Stable clones of ZNF331 overexpression were established.ZNF331 showed no significant effect on cell proliferation of HCT 116/p53 +/+, but inhibited cell proliferation of HCT116/p53 -/-(P<0.01).ZNF331 could interact with p53,dose-dependently inhibit the transcriptional activity of p53 and downregulate the mRNA levels of pro-apoptotic p53 target genes, Puma and p53AIP1 (P<0.05).ZNF331 could suppress p53-induced apoptosis(P <0.01).Conclusion The influence of ZNF331 overexpression on colon cancer cell proliferation is dependent on p 53 status.ZNF331 overexpression can suppress colon cancer cell apoptosis by interacting with p 53 and inhibiting the transcriptional activity of p 53.关键词
ZNF331/结肠肿瘤/细胞增殖/细胞凋亡/p53/HCT116细胞Key words
ZNF331/colonic neoplasms/cell proliferation/cell apoptosis/p53/HCT116 cells分类
医药卫生引用本文复制引用
尹钰,张秀园,令吉明,耿云峰,王伟龙,鞠吉雨,赵春玲,田春艳..ZNF331过表达对结肠癌细胞增殖与凋亡的影响[J].军事医学,2018,42(1):38-43,6.基金项目
国家自然科学基金资助项目(81572578,81541093) （81572578,81541093）
山东省自然科学基金项目(ZR2015HM028,ZR2015HL075) （ZR2015HM028,ZR2015HL075）
