军事医学2018，Vol.42Issue(1)：49-52,4.DOI:10.7644/j.issn.1674-9960.2018.01.012
12-氧-十四烷酰佛波醇-13-癸酰酯对小鼠肠道急性放射损伤的保护作用及机制研究
Protective effects and possible mechanism of 12-O-tetradecanoylophorbol-13-decano-ate against acute intestinal radiation injury of mice
摘要
Abstract
Objective To investigate the effect of 12-O-tetradecanoylophorbol-13-decanoate(TPD)on protection against acute intestinal radiation injury of mice and the possible mechanism.Methods Twenty female BALB/c mice aged 6-8 weeks were divided by random number table method into the control group and TPD groups(25,50,and 100 μg/kg). A radiation-damaged model of mice was irradiated by 10 Gy 60Co γ-rays,while the TPD groups were pretreated for 3 d with caudal vein injection before irradiation.The survival time of 20 days and the number of crypts at 3.5 days after irradiation were detected.Rat intestinal epithelial cells(IEC-6)were treated with 1 nmol/L TPD for 12 h before irradiation with 10 Gy 60Co γ-rays,and CCK-8 was used to detect the capability of cell proliferation at 0,1,2,3 and 4 d after irradiation. Results The mice in the control group survived for an average of 4.2 days,compared to 10 days in the optimal TPD group (100 μg/kg).The average number of crypts in the control group and the best TPD group was 11.0 ±1.3 and 35.1 ±1.9 respectively.The proliferation activity of IEC-6 was measured for four consecutive days.The average D value of the TPD groups was significantly higher than that of control.Conclusion TPD has a protective effect against acute intestinal radiation injury, and its protective mechanism may be achieved by promoting intestinal crypt cell proliferation and increasing the number of crypts in the intestine.关键词
12-氧-十四烷酰佛波醇-13-癸酰酯(TPD)/小鼠/辐射损伤/小肠/急性肠放射损伤/肠隐窝/放射疗法/γ射线/保护机制Key words
12-O-tetradecanoylophorbol-13-decanoate(TPD)/mice/radiation injuries/intestine/small/acute intestinal radiation injury/intestinal crypt/radiotherapy/Gamma rays/protection mechanism分类
医药卫生引用本文复制引用
柳茜,王志红,周宏伟,姚毅冰,刘默,周敏航,程莉,孙君重..12-氧-十四烷酰佛波醇-13-癸酰酯对小鼠肠道急性放射损伤的保护作用及机制研究[J].军事医学,2018,42(1):49-52,4.基金项目
国家自然科学基金青年资助项目(81601984) （81601984）
北京市自然科学基金青年资助项目(7164300) （7164300）
