军事医学2018，Vol.42Issue(1)：53-56,4.DOI:10.7644/j.issn.1674-9960.2018.01.013
检测4种血流感染病原体的靶向序列富集与测序相结合技术方法评价
Evaluation of target-enrichment accompanied by next-generation sequencing for detection of four pathogens in bloodstream infections
摘要
Abstract
Objective To establish an Ampliseq method that combines target-enrichment and the next-generation sequencing for simultaneous detection of Staphylococcus aureus, S.epidermidis, Klebsiella pneumoniae and Listeria monocytogenes in order to provide a fast and accurate means to detect pathogens in bloodstream infections.Methods A method to evaluate the LOD,specificity and sensitivity by constructing simulated samples spiked in known pathogens was established.Results and Conclusion Target-enrichment Ampliseq showed good sensitivity and specificity,and the limit of detection(LOD)was as low as 101CFU/ml.The sensitivity was 95.38%,the specificity was 95.45%and the Kappa value was between 0.839 -1.000.This method can detect S.aureus,S.epidermidis,K.pneumoniae and L.monocytogenes simultaneously in one reaction within 15 hours.关键词
血流感染/Ampliseq/二代测序/目标序列富集Key words
bloodstream infection/Ampliseq/the next-generation sequencing/target-enrichment分类
医药卫生引用本文复制引用
李倩,刘鹏,张彦,张超,黄文华,郑玉玲,王洪权,姜永强..检测4种血流感染病原体的靶向序列富集与测序相结合技术方法评价[J].军事医学,2018,42(1):53-56,4.基金项目
北京市自然科学基金资助项目(7172155) （7172155）
国家自然科学基金资助项目(81772144) （81772144）
