军事医学2018，Vol.42Issue(1)：57-59,3.DOI:10.7644/j.issn.1674-9960.2018.01.014
基于原代小脑颗粒细胞的A型肉毒毒素活性分析法的建立
Evaluation of activity of botulinum neurotoxin A based on primary cerebellar granu-losa cells
摘要
Abstract
Objective To isolate and culture the primary cerebellar granulosa cells(CGNs)of SD rats and evaluate the activity of botulinum neurotoxin A(BoNT/A)based on CGNs.Methods CGNs of 6-to 8-days-old SD rats were isolated and cultured.After 5-7 d,the cells were treated with BoNT/A.The activity of the toxin was evaluated with immunofluo-rescence,and the relationships between the activity and dose of the toxin were analyzed with Western blotting.Results and Conclusion CGNs Of SD rats were successfully cultured,a method for evaluating the activity of BoNT/A was established at the level of primary nerve cells,and the relationships between the activity and dose of toxin were analyzed.This study provides a tool for further detailing the biochemical mechanism of BoNT /A.关键词
A型肉毒毒素/原代小脑颗粒细胞/活性分析Key words
botulinum neurotoxin A/primary cerebellar granulosa cells/activity analysis分类
医药卫生引用本文复制引用
王建新,李崭,何志利,黄洁,李涛,王慧..基于原代小脑颗粒细胞的A型肉毒毒素活性分析法的建立[J].军事医学,2018,42(1):57-59,3.基金项目
国家重点基础研究发展计划资助项目(2015CB554202) （2015CB554202）
