军事医学2018，Vol.42Issue(1)：60-63,4.DOI:10.7644/j.issn.1674-9960.2018.01.015
睡眠剥夺对大鼠肝脏生物钟基因表达水平的影响
Effects of sleep deprivation on liver circadian clock gene expression in rats
摘要
Abstract
Objective To investigate the effects of 72 h sleep deprivation(SD)on circadian clock gene expression in the rat liver.Methods Twelve rats were randomly divided into control group and SD group.An SD instrument was used to deprive the rats′sleep for 72 h.Then the abdominal cavity was exposed to obtain liver,and the expression of clock genes was detected by RT-PCR and Western blotting analysis, respectively.Results Compared with the control group, the mRNA levels of clock,npas2 and rev-erbαstrikingly decreased in the livers of the SD group rats.However,per1,per2 and rorαmRNA levels obviously increased.bmal1 and cry1 mRNA expression hardly changed in the control and SD groups. Meanwhile,the protein levels of liver BMAL1,CLOCK,NPAS2,CRY1 and REV-ERBαwere significantly down-regulated and PER1,PER2 and RORαprotein levels were up-regulated in SD group compared with control group.Conclusion 72 h SD can result in abnormal expressions of several circadian clock genes in the rat liver at both transcriptional and translational levels.关键词
睡眠剥夺/昼夜节律/生物钟/基因表达/肝脏Key words
sleep deprivation/circadian rhythm/circadian clock/gene expression/liver分类
医药卫生引用本文复制引用
邢陈,顾晔,徐秀段,邹书仙,胡永亮,胡美茹,宋伦..睡眠剥夺对大鼠肝脏生物钟基因表达水平的影响[J].军事医学,2018,42(1):60-63,4.基金项目
军队"十三五"重大科研资助项目(AWS17J014) （AWS17J014）
军队"十三五"重点科研资助项目(DWS17J025) （DWS17J025）
军队"十二五"重大科研资助项目(AWS14J011) （AWS14J011）
北京市自然科学基金青年资助项目(7164287) （7164287）
细胞应激生物学国家重点实验室(厦门大学)开放基金资助项目(SKLCSB2016KF010) （厦门大学）
