临床口腔医学杂志2018，Vol.34Issue(5)：259-262,4.DOI:10.3969/j.issn.1003-1634.2018.05.002
rnc基因调控变形链球菌蔗糖依赖性黏附能力的机制研究
Regulation mechanism of rnc gene on sucrose-dependent cell adherence of Streptococcus mutans
摘要
Abstract
Objective:The aim of the current study was to verify the involvement of rnc gene in the sucrose-depend-ent cell adherence of Streptococcus mutans(S.mutans),and to analyze its functional roles.Methods:An rnc knockout mutant (Smurnc)was constructed by PCR ligation mutagenesis in S.mutans UA159.Sucrose-dependent cell adherence assay was performed to compare the adhesion ability of Streptococcus mutans UA159 and Smurnc.RT-qPCR was used to analyze the effects of rnc knockout on the expression of the sucrose-dependent cell adherence related genes at the transcriptional level. Results:The sucrose-dependent adhesion and the biofilm formation decreased in Smurnc.Expression of the sucrose-depend-ent cell adherence related genes gbpB,gtfB,gtfD and dexA had been significantly upregulated,gbpC and gbpD of the rnc knockout mutant had been significantly downregulated.Conclusion:Based on these findings,it was established that rnc deple-tion impaired sucrose-dependent adherence of S.mutans UA159.rnc gene may regulate the expression of sucrose-dependent adherence related genes and involve in the cariogenecity of Strptococcus mutans.关键词
变形链球菌/rnc基因/蔗糖依赖性黏附/生物膜Key words
Streptococcus mutans/rnc gene/Sucrose-dependent cell adherence/Biofilm分类
医药卫生引用本文复制引用
张茹,雷蕾,杨英明,胡涛..rnc基因调控变形链球菌蔗糖依赖性黏附能力的机制研究[J].临床口腔医学杂志,2018,34(5):259-262,4.基金项目
国家自然科学基金(81300878,81771068) （81300878,81771068）
北京市自然科学基金(7144214) （7144214）
北京市医院管理局青年人才培养青苗计划资助(QML20151403) （QML20151403）
