临床口腔医学杂志2018，Vol.34Issue(5)：267-270,4.DOI:10.3969/j.issn.1003-1634.2018.05.004
miR-96在口腔鳞状细胞癌中的表达及作用的相关研究
Expression and role of miR-96 in oral squamous cell carcinoma
许璐 1郭松2
作者信息
- 1. 新汶矿业集团公司中心医院 肿瘤科 山东 泰安 271233
- 2. 新汶矿业集团公司中心医院 口腔科 山东 泰安 271233
- 折叠
摘要
Abstract
Objective:To explore the difference in miR-96 expression in human oral squamous cell carcinoma(OS-CC)tissue specimens and cell line.Methods:miR-96 expression in human OSCC tissue specimens and cell line was detected using real-time fluorescent quantitative PCR(qRT-PCR),and the correlation of miR-96 expression with the clinicopathologi-cal features of patients was analyzed.miR-96 inhibitor was transfected in vitro to the human OSCC cell Tca8113,and changes in miR-96 expression in cells after transfection were detected using qRT-PCR.Meanwhile,changes in cell proliferation and apoptosis after transfection were determined using CCK-8 assay and flow cytometry.Results:qRT-PCR results indicated that miR-96 showed aberrantly high expression in human OSCC cell Tca8113,as well as OSCC tissue specimens(P<0.01).Fur-ther statistical analysis suggested that miR-96 expression in OSCC tissue specimens was closely related to clinical stage and lymph node metastasis(P<0.01).Moreover,miR-96 expression level had remarkably reduced(P<0.01)in Tca8113 cell after transfection with miR-96 inhibitor,cell proliferation capacity had markedly lowered(P<0.01),and cell apoptosis rate had evidently enhanced(P<0.01).Conclusion:miR-96 shows aberrantly high expression in OSCC tissue specimens and cell line.Down-regulating miR-96 expression can apparently suppress OSCC cell proliferation,and enhance its apoptosis.关键词
口腔鳞状细胞癌/miR-96/增殖/凋亡Key words
Oral squamous cell carcinoma/miR-96/Proliferation/Apoptosis分类
生物科学引用本文复制引用
许璐,郭松..miR-96在口腔鳞状细胞癌中的表达及作用的相关研究[J].临床口腔医学杂志,2018,34(5):267-270,4.
