临床口腔医学杂志2018，Vol.34Issue(5)：271-274,4.DOI:10.3969/j.issn.1003-1634.2018.05.005
一种双因子控释牙周再生支架材料的构建
Preparation of dual factor-released scaffolds for periodontal regeneration
摘要
Abstract
Objective:To prepare dual factor-loaded scaffolds for controlled release of platelet-derived growth factor (PDGF)and simvastatin(SMV)and to evaluate the release properties of this formulation.Methods:SMV was encapsulated in collagen/polycaprolactone membranes using the electrospining method and surface wettability of the membranes was deter-mined by static contact angle measurement.Then,PDGF was assembled onto the surface of electrospun membranes using the electrostatic layer-by-layer coating method.The in vitro release profiles of PDGF and SMV were determined by an enzyme linked immunosorbent assay(ELISA)kit and an ultraviolet-visible spectrometer,respectively. Results:Reduction of water contact angle showed good hydrophilicity of the polycaprolactone membrane,which increased in the presence of collagen.The in vitro release analysis demonstrated a sequential release profile,which was characterized by the fast release of PDGF with sustained concentration from day-3 to day-13,followed by the slow release of SMV with sustained concentration from day-6 to day-21.Conclusion:Dual factor-released membrane scaffolds were successfully fabricated by encapsulating PDGF and SMV with distinct shell-core structure.The combination and sequential release of PDGF and SMV could be application in periodon-tal tissue engineering.关键词
静电纺丝/血小板源性生长因子/辛伐他汀/自组装/缓释Key words
Electrospinning/Platelet-derived growth factor/Simvastatin/Layer-by-layer/Controlled release分类
医药卫生引用本文复制引用
赵丙姣,陈静,刘月华..一种双因子控释牙周再生支架材料的构建[J].临床口腔医学杂志,2018,34(5):271-274,4.基金项目
国家自然科学基金(81701011,81470768) （81701011,81470768）
上海市自然科学基金(15ZR1435900) （15ZR1435900）
上海市青年科技英才扬帆计划项目(17YF1416500) （17YF1416500）
