林业科学2018，Vol.54Issue(3)：19-28,10.DOI:10.11707/j.1001-7488.20180303
杂交鹅掌楸体胚再生植株淹水胁迫下叶片超微结构及光合特性变化
Changes on Leaf Chloroplast Ultrastructure and Photosynthetic Characteristics of Liriodendron sino-americanum Somatic Embryo Regeneration Seedlings under Waterlogging Stress
摘要
Abstract
[Objective] In this study,we investigated the changes of the phenotype,chloroplast ultrastructure and photosynthetic characteristics of Liriodendron sino-americanum under waterlogging stress,to reveal the response mechanism of L.sino-americanum in waterlogged stress and thereby provide a theoretical basis for promotion of cultivation and application in waterlogged districts.[Method] L.sino-americanum somatic embryo regeneration seedlings with 3-5 leaves were subjected to sterile waterlogging treatment for various durations (1d,3d,5d,7d,and 2d recovery after 7days waterlogging),and the effects of the treatments on phenotype,chloroplast ultrastructure and photosynthetic characteristics were investigated.[Result] The results indicated that:1) The longer waterlogging treatment led to more severe damages to L.sino-americanum seedlings including curled and yellow leaves,or even leaves fell off from the base.However,the apical buds had no obvious changes,which could be due to the defense mechanism initiated by L.sino-americanum under waterlogging stress.2) Waterlogging stress resulted in swelled chloroplasts and severe damages to the chloroplast ultrastructure of L.sino-americanum including degradation of chloroplast membrane system,loose and disordered arrangement of grana and stoma lamella,accumulation of starch grains,and osmiophilic granules density increased and and gathered together at the late stage of stress.3) The net photosynthetic rate,stomatal conductance and transpiration rate were decreased whereas intercellular CO2 concentration was increased slowly.The electron transport rate,photochemical quenching coefficient,photochemical efficiency and photochemical actual quantum yield in photosynthesizerⅡ showed a significant decline.The photosynthetic characteristics were partially restored after 2 days recovery treatment after 7d waterlogging stress.These data suggested that waterlogging stress caused serious damage in photosynthesizer Ⅱ activity center,and L.sino-americanum reduced gas exchanges by closing the stomata.[Conclusion] Taken together,waterlogging stress can cause the damages of chloroplast ultrastructure of L.sino-americanum,and then damage the photosynthesizer Ⅱ activity center,resulting in a decrease in leaf photosynthetic capacity and inhibition on growth and development of L.sinoamericanum seedlings,but the seedlings may obtain to certain degree resistance under short-term waterlogging stress.关键词
杂交鹅掌楸/体胚再生植株/淹水胁迫/超微结构/光合特性Key words
Liriodendron sino-americanum/embryo regeneration seedlings/waterlogging stress/chloroplastic ultrastructure/photosynthetic characteristics分类
农业科技引用本文复制引用
周艳威,陈金慧,鲁路,成铁龙,杨立明,施季森..杂交鹅掌楸体胚再生植株淹水胁迫下叶片超微结构及光合特性变化[J].林业科学,2018,54(3):19-28,10.基金项目
“十二五”国家科技支撑计划课题“珍贵用材树种新品种选育技术研究(2012BAD01B005)” （2012BAD01B005）
国家863计划(2013AA102705) （2013AA102705）
教育部高等学校博士学科点专项科研基金(SRFDP,20113204130002) （SRFDP,20113204130002）
江苏省高校自然科学基金(13KJA220001) （13KJA220001）
南京林业大学南方现代林业协同创新中心和江苏省高校优势学科建设工程资助项目(PAPD). （PAPD）
