南京工业大学学报（自然科学版）2018，Vol.40Issue(3)：31-37,7.DOI:10.3969/j.issn.1671-7627.2018.03.006
矢车菊素与矢车菊素-3-O-葡萄糖苷对H2O2诱导HepG-2细胞氧化应激保护作用的比较
Comparison of protective effects of cyanidin and cyanidin-3-O-glucoside on oxidative stress induced by H2O2 in HepG-2 cell model
摘要
Abstract
Protection effects of cyanidin(Cy)and its derivate cyanidin-3-O-glucoside(C3G)on cellular oxidative stress induced by H2O2on HepG-2 cell line were compared.The cell viabilities were detected by thiazolyl blue tetrazolium bromide(MTT)assay and the antioxidant protection were evaluated by total antioxidant capacity(T-AOC),the activity of superoxide dismutase(SOD),concentration of glutathione (GSH)and malondialdehyde(MDA)in cell.Results showed that two anthocyanins had no significant toxic effects on cultured HepG-2 cells under the concentration range of 0-0.5 mmol/L.Compared with H2O2control group,both anthocyanins could increase the levels of T-AOC and SOD,concentration of GSH,and decrease the concentration of MDA in HepG-2 cells at different concentration of 50,200 and 400 μmol/L.The antioxidant capacity positively correlated with the concentration of anthocyanins.The antioxidant effect of Cy was significantly higher than that of C3G.The study could provide a theoretical basis for further understanding the structure-activity relationship of anthocyanin in vivo.关键词
矢车菊素/矢车菊素-3-O-葡萄糖苷/H2O2/氧化应激模型Key words
cyanidin/cyanidin-3-O-glucoside/hydrogen peroxide/oxidative stress model分类
化学化工引用本文复制引用
张楚阳,张珂嘉,刘琴..矢车菊素与矢车菊素-3-O-葡萄糖苷对H2O2诱导HepG-2细胞氧化应激保护作用的比较[J].南京工业大学学报（自然科学版）,2018,40(3):31-37,7.基金项目
江苏省高校自然科学研究重大项目(11KJA550001) （11KJA550001）
江苏高校优势学科建设工程 （）
