南京农业大学学报2018，Vol.41Issue(3)：456-464,9.DOI:10.7685/jnau.201709023
施用氨基酸有机肥对黄瓜产量及土壤生物学性状的影响
Effects of amino acid organic fertilizer on cucumber yield and soil biological characters
摘要
Abstract
[Objectives]Amino acid liquid,which is hydrolyzed from animal carcasses by concentrated sulfuric acid under high temperature,compounded with manure compost was used to develop a novel amino acid organic fertilizer. This is one of important ways to achieve the goal of bio-safety disposal and utilization of dead livestock and poultry to ensure the sustainable development of farmed animal industries. This study is designed to evaluate the effects of this amino acid organic fertilizer application on cucumber yield and soil biological properties. [Methods]The effects of continuous application of the novel product on greenhouse cucumber yield,soil physicochemical properties,nitrogen cycling microorganisms and soil enzyme activity were studied by real-time PCR and other technologies. [Results]Field experiment showed that the cucumber yield in amino acid organic fertilizer application treatment (AOF)increased by 48.2%,29.5% and 30.6% when compared with non-fertilization control(CK),fertilization treatments with appli-cation of only chemical fertilizer(CF)and common organic fertilizer(OF),respectively. Compared to CK,application of amino acid organic fertilizer could significantly increase soil pH value and the contents of total carbon,ammonium nitrogen,nitrate nitrogen and available phosphorus,but only display small differences compared to other fertilization treatments(CF and CK). As revealed by real time PCR,the soil sampled from AOF exhibited a significantly higher abundance of ammonia-oxidizing bacteria than that in soil collected from CK while exhibited a significantly lower abundance than that in soil collected from CF and OF. Meanwhile,soil collected from AOF displayed a significantly higher abundance of denitrifying nirK gene compared to soil sampled from CK but exhibited a significantly lower abundance compared to OF. Also,soil collected from AOF showed a significantly higher abundance of nirS gene compared to CK and CF. Amino acid organic fertilizer treated soil showed highest activities of catalase and phosphatase when compared to non-fertilization control and fertilization treatments.Also,amino acid organic fertilizer treated soil showed a signifi-cantly higher activity of urease compared to CK but lower activities compared to CF and OF treatments. Moreover,sucrase activity was significantly higher for AOF treatment than that for CK with no significant difference comparing with CF and CK. [Conclusions]All results illustrated that the application of amino acid organic fertilizer could promote the cucumber yield probably through manipulating soil physicochemical properties,enhancing soil enzyme activity and nitrification,and then improving roots utilization of available nutrients and free amino acid in soil.关键词
氨基酸有机肥/黄瓜/产量/土壤酶活性/土壤养分/氮素循环微生物Key words
amino acid organic fertilizer/cucumber/yield/soil enzyme activity/soil nutrient/nitrogen cycling microorganism分类
农业科技引用本文复制引用
吕娜娜,沈宗专,王东升,刘红军,薛超,李荣,沈其荣..施用氨基酸有机肥对黄瓜产量及土壤生物学性状的影响[J].南京农业大学学报,2018,41(3):456-464,9.基金项目
国家重点研发计划项目(2016YFE0101100,2017YFD0202101) （2016YFE0101100,2017YFD0202101）
中央高校基本科研业务费专项资金(KJQN201746) （KJQN201746）
国家公益性行业(农业)科研专项(201503110) （农业）
国家自然科学基金项目(31601836) （31601836）
中国博士后科学基金项目(2016M590469) （2016M590469）
