水产学报2018，Vol.42Issue(5)：625-632,8.DOI:10.11964/jfc.20170410801
黄颡鱼FZD家族4个基因的克隆、组织表达及对铜的响应
Molecular characterization and tissue distribution of Frizzled (FZD) in yellow catfish(Pelteobagrus fulvidraco)by copper exposure
摘要
Abstract
Four complete FZD cDNA sequences,designated fzd2,fzd3a,fzd4 and fzd10,were amplified by RT-PCR and RACE approaches from Pelteobagrus fulvidraco.The validated cDNAs encoding for fzd2,fzd3a,fzd4 and fzd10 were 2 176,3 243,2 509 and 3 021 bp in length,encoding peptides of 551,695,550 and 586 amino acid residues, respectively. The amino acid alignment and phylogenetic analysis revealed that Fzd sequences were relat-ively conserved among different vertebrates,and fzds of P.fulvidraco were more closely related to those of Ictalur-us punctatus.All fzds mRNAs were expressed in all tested tissues(brain,spleen,kidney,gill,heart,muscle,mes-enteric fat,liver and ovary),but at variable levels.On day 28,fzd3a mRNA levels increased with increasing water-borne Cu concentration, but mRNA expression showed no significant differences among the three groups. On day 56,fzd2 mRNA levels were the highest in 30 μg Cu/L group and showed no significant differences between other two groups,and mRNA levels of fzd3a,fzd4 and fzd10 showed no significant differences among three groups.The present study will be helpful to understanding the physiological functions of fzd genes.关键词
黄颡鱼/FZD/分子特征/组织表达/铜Key words
Pelteobagrus fulvidraco/Frizzled(FZD)/molecular characterization/tissue distribution/copper exposure分类
生物科学引用本文复制引用
张丽晗,罗智,有文静,李丹丹,吴坤,潘亚雄..黄颡鱼FZD家族4个基因的克隆、组织表达及对铜的响应[J].水产学报,2018,42(5):625-632,8.基金项目
国家自然科学基金(31422056) （31422056）
National Natural Science Foundation of China(31422056) （31422056）
