水产学报2018，Vol.42Issue(5)：633-645,13.DOI:10.11964/jfc.20170510846
中国明对虾Dscam基因的克隆及其在免疫致敏(类免疫)诱导反应中的表达分析
Cloning and expression analysis of the Dscam gene during the inducing immune priming (Quasi-immune) response in Fenneropenaeus chinensis
摘要
Abstract
To explore the function of Dscam gene of Chinese shrimp Fenneropenaeus chinensis(FcDscam)dur-ing the immune priming response, we cloned the cDNA sequence using the rapid amplification of cDNA ends (RACE),and then analyzed the features of this gene based on bioinformatics software.The full length of FcD-scam cDNA is 6624 bp,with a 5′-untranslated region(UTR)of 171 bp,a 3′-untranslated region(UTR)of 459 bp, and an opening reading frame(ORF)of 5 994 bp,and it encoded 1 996 amino acids.The protein encoded by FcD-scam contains 1 signal peptide,1 cytoplasmic tail,1 transmembrane domain,6 of fibronectin type (FN)and 10 of immunoglobulin(Ig).As shown by the homology search and neighbor-joining phylogenetic tree,FcDscam gene had the highest homology with that of Litopenaeus vannamei(92.4%).To induce the immune response,test anim-als were first fed with heat-inactivated WSSV bait for six days and infected again 12 days later. Samples were col-lected at 0, 6 and 12 d of the first-time infection and 12, 24, 48, 72 and 168 h of the second-time infection, respect-ively,for analysing the mRNA expression level of FcDscam by real-time PCR.On the 12th day of the first infec-tion, its expression level in induced infection group began to increase, significantly different from those in the neg-ative control group and the non-induced infection group. After 24 h of the second-time infection, its expression level in the infection group reached a maximum, significantly different from those of the negative control group and the non-induced infection group, and the expression level started to decline at the 48 h. Our research demon-strated the existence of the Dscam gene in F.chinensis,and its important role in the process of immune sensitiza-tion.关键词
中国明对虾/Dscam基因/免疫致敏/表达分析Key words
Fenneropenaeus chinensis/Dscam/immune priming/expression analysis分类
生物科学引用本文复制引用
曹家旺,孟宪红,孔杰,史晓丽,栾生,罗坤,董丽君,陈宝龙..中国明对虾Dscam基因的克隆及其在免疫致敏(类免疫)诱导反应中的表达分析[J].水产学报,2018,42(5):633-645,13.基金项目
中国水产科学研究院黄海水产研究所基本科研业务费专项(20603022017001) （20603022017001）
国家自然科学基金(31372523) （31372523）
泰山学者种业团队项目 （）
国家现代农业产业技术体系(CARS-48) （CARS-48）
Central Public-interest Scientific Institution Basal Research Fund,Yellow Sea Fisheries Re-search Institute(20603022017001) （20603022017001）
National Natural Science Foundation of China(31372523) （31372523）
The Taishan Scholar Program for Seed Industry （）
China Agriculture Research System(CARS-48) （CARS-48）
