水产学报2018，Vol.42Issue(5)：653-662,10.DOI:10.11964/jfc.20170110686
雌激素拮抗剂对三疣梭子蟹卵巢发育及相关基因表达的影响
Effects of estrogen antagonist on ovarian development and expression of related genes to ovarian development of Portunus trituberculatus
摘要
Abstract
Estrogen plays an important role during the regulation of ovarian development in crustaceans, and tamoxifen (TAM) is a commonly used estrogen antagonist for the studying of molecular mechanism between es-trogen and ovarian development of crustacean. This study was designed to investigate the effect of estrogen antag-onist on survival rate, gonadosomatic index (GSI), hepatosomatic index (HSI), and expressions of the related genes to ovarian development of female Portunus trituberculatus by in vivo experiment,culture experiment and quantit-ative PCR (qPCR). The results showed that different concentrations of TAM had no significant effects on the sur-vival rate and HSI among all treatments, but could suppress GSI. The results of qPCR showed that TAM had signi-ficant effects on the expression levels of Vg (vitellogenin), ERR (estrogen related receptor), RXR (retinoid X re-ceptor)and EcR(ecdysone receptor)genes in different tissues of P.trituberculatus.The expression levels of Vg gene in ovary and hepatopancreas were obviously inhibited by TAM.For ERR gene,low concentration of TAM (6.7 and 13.4 μg/g)could promote the ERR transcription in the ovary and thoracic ganglion,while different from the results of low concentration,the significant decrease was found on the ERR mRNA levels in the most ex-amined tissues from the high concentration of TAM treatment (20 μg/g) compared to the control treatment. The ex-pression profiles of EcR affected by TAM were similar to ERR in ovary and hepatopancreas.In addition,the low concentration of TAM could promote the expression of RXR gene in ovary,but no significant differences were found on RXR expression levels in the hepatopancreas.In conclusion,these results suggested that TAM could in-hibit the Vg expression levels,possibly resulting in the low GSI of female P.trituberculatus,and ERR and EcR might be involved in regulatory processes of estrogen or exogenous estrogen antagonist on ovarian development.关键词
三疣梭子蟹/卵巢发育/雌激素拮抗剂/基因表达Key words
Portunus trituberculatus/ovarian development/estrogen antagonist/expression analysis分类
生物科学引用本文复制引用
陆洋,吴旭干,柳梅梅,巩杰,成永旭..雌激素拮抗剂对三疣梭子蟹卵巢发育及相关基因表达的影响[J].水产学报,2018,42(5):653-662,10.基金项目
国家自然科学基金(41276158,41606169) （41276158,41606169）
上海市自然科学基金(12ZR1413000) （12ZR1413000）
上海市科技兴农重点推广项目(农科推字(2016)第1-18号) （农科推字(2016）
上海市高峰学科建设项目(2015-62-0908) （2015-62-0908）
浙江省东海海水养殖产业升级协同创新中心项目 （）
National Natural Science Foundation of China(41276158,41606169) （41276158,41606169）
Shanghai Natural Sci-ence Foundation Project(12ZR1413000) （12ZR1413000）
Key Research and Extension Project from Shanghai Municipal Agricul-ture Commission Project(2016-1-18) （2016-1-18）
Shanghai Universities Top Disciplines Project of Fisheries(2015-62-0908) （2015-62-0908）
Collaborative Innovation Project for Mariculture industry in East China Sea （）
