水产学报2018，Vol.42Issue(5)：663-672,10.DOI:10.11964/jfc.20170110688
菲律宾蛤仔不同发育时期及成体不同组织中内参基因筛选
The selection of reference genes in different development stages and different tissues of Ruditapes philippinarum
摘要
Abstract
To select the reference genes applicable for 17 development stages and 7 tissues of Ruditapes philip-pinarum,expression stabilities of elongation factor 1 alpha gene(EF1A),TATA-box binding protein gene(TBP), histone gene(HIS),cytochrome b5 gene(CYTB5),ubiquitin-conjugating enzyme gene(UCE),ribosomal protein L8 gene(RPL8),ribosomal protein S23 gene(RPS 23),ribosomal protein L2 gene(RPL 2),cytochrome C gene (CYTC),growth factor receptor-bound protein 2 gene(GFRP2),β-actin gene(ACT)and tubulin gene(TUB)were then evaluated with three algorithms—geNorm, NormFinder and ΔCt methods, respectively. The results suggest that CYTC,CYTB5 and RPS23 were the optimal reference gene combination for different development stages;CYTB5 and GFRP2 were recommended for qRT-PCR normalization of different tissues.The ACT was proved to be the most unstable in different development stages and different tissues of R.philippinarum.关键词
菲律宾蛤仔/内参基因/实时荧光定量PCR/不同发育时期/不同组织Key words
Ruditapes philippinarum/reference genes/qRT-PCR/different development stages/different tissues分类
生物科学引用本文复制引用
牟政强,闫路路,王化敏,霍忠明,闫喜武..菲律宾蛤仔不同发育时期及成体不同组织中内参基因筛选[J].水产学报,2018,42(5):663-672,10.基金项目
现代农业产业技术体系建设专项(CARS-48) （CARS-48）
Earmarked Fund for Modern Agro-industry Technology Research System(CARS-48) （CARS-48）
