水产学报2018，Vol.42Issue(5)：766-776,11.DOI:10.11964/jfc.20170410790
不同糖及糖水平对松浦镜鲤养殖过程中糖代谢相关基因表达的影响
Effects of different carbohydrates and different carbohydrate levels on mRNA expression of glucose metabolism related genes in different feeding stages of Songpu mirror carp(Cyprinus carpio)
摘要
Abstract
This experiment was aimed to study the effect of different carbohydrates and different carbohydrate levels on mRNA expression of glycolytic enzyme(glucose kinase,GK;hexokinase,HK),gluconeogenesis en-zyme(Glucose-6-phosphatase,G6Pase;phosphoenolpyruvate carboxykinase,PEPCK),glucose metabolism re-lated genes(Insulin-like Growth Factor-Ⅰ,IGF-Ⅰ;Growth Hormone Receptor,GHR)in different feeding stages of Songpu mirror carp(Cyprinus carpio).Four experimental diets were formulated to contain two carbohydrates (starch and glucose) diets with two levels (250 g /kg and 500 g/kg) (Low level starch group: LS; High level starch group: HS; Low level glucose group: LG; High level glucose group: HG) two carbohydrates (glucose and starch) and two carbohydrate levels (25% and 50%) (LS/HS/LG/HG) using 2×2 double factor experimental design, 420 Songpu mirror carp(mean weight=[8.30±0.15]g)were randomly divided into 4 dietary treatments with 3 triplic-ates and 35 fish per triplicate and were reared for 7 weeks. The results showed that in different feeding stages, the mRNA expression levels of HK gene and IGF-Ⅰgene in different experimental groups were basically in the same trend.The mRNA expression levels of HK gene at feeding 1W was significantly increased and then decreased.The mRNA expression level of IGF-Ⅰgene was significantly increased at feeding 3W.IGF-Ⅰgene expression in-creased significantly at feeding 3W.In different feeding stages,the mRNA expression levels of GK,PEPCK, G6Pase and GHR genes were affected by carbohydrate levels and carbohydrate types.At low carbohydrate level (LS group and LG group), the mRNA expression levels of these genes showed the same trend. The mRNA expres-sion levels of GK and GHR genes were significantly higher at feeding 1W and then began to decline.The mRNA expression level of PEPCK gene was the highest at feeding 3W.The mRNA expression level of G6Pase genes were significantly higher at feeding 1W, then began to decline and showed a rising trend at feeding 7W. At high carbohydrate levels,the mRNA expression level of GK gene in HS group was not significantly different at differ-ent feeding stages.But the mRNA expression level of GK gene in HG group was increased significantly at feeding 1W.The mRNA expression level of PEPCK gene in HS group and HG group had no significant difference at dif-ferent feeding stages.The mRNA expression level of G6Pase gene in HS group had no significant difference at different feeding stages.But the mRNA expression level of G6Pase gene in HG group was increased significantly at feeding 3W.The mRNA expression level of GHR gene in HG group had no significant difference at different feeding stages,while the mRNA expression level of GHR gene in HS group was increased significantly at feeding 3W.In the early feeding stage(1W),the mRNA expression level of HK gene was significantly promoted by high carbohydrate diet.While,the mRNA expression levels of PEPCK,G6Pase and GHR genes were suppressed by high carbohydrate diet. In conclusion, this study investigated the effects of different carbohydrates and carbo-hydrate levels on the expression trend of enzymes and genes involved in glucose metabolism at different feeding stages. It provides a theoretical basis for the application of carbohydrate in feed.关键词
松浦镜鲤/葡萄糖/淀粉/糖酵解酶/糖异生酶/基因表达Key words
Songpu mirror carp(Cyprinus carpio)/glucose/starch/glycolytic enzyme/gluconeogenesis enzyme/gene expression分类
生物科学引用本文复制引用
李晋南,王常安,王连生,赵志刚,罗亮,徐奇友..不同糖及糖水平对松浦镜鲤养殖过程中糖代谢相关基因表达的影响[J].水产学报,2018,42(5):766-776,11.基金项目
中央级公益性科研院所基本科研业务费专项(HSY201605) （HSY201605）
国家自然科学基金(31702353) （31702353）
现代农业产业技术体系建设专项(CARS-45) （CARS-45）
Central-level Non-profit Science Research Institutes Special Funds(HSY201605) （HSY201605）
National Natural Science Foundation of China(31702353) （31702353）
China Agriculture Research System(CARS-45) （CARS-45）
