水产学报2018,Vol.42Issue(5):777-786,10.DOI:10.11964/jfc.20170510849
不同碳水化合物和蛋白质水平膨化饲料对大规格草鱼生长、肠道消化酶及血清指标的影响
Effects of different dietary carbohydrate and protein levels on growth, intestinal digestive enzymes and serum indexes in large-size grass carp(Ctenopharyngodon idella)
摘要
Abstract
A 16-week feeding experiment was conducted to study the effects of different dietary carbohydrate and protein levels on growth, body composition, intestinal digestive enzyme activity and serum indexes of large-size grass carp(Ctenopharyngodon idella)[initial body weight(398.6±5.9 g)].Seven extruded diets with different levels of carbohydrate (C) and protein (P) (C31P30, C34P28, C37P26, C40P24, C43P22, C46P20, C49P18) were made. Experimental results showed that with protein levels decreasing and carbohydrate levels increasing, weight gain rate decreased significantly, protein efficiency significantly increased, hepatosmatic index, viscera index, in-testinosomatic index and the fat content of muscle and liver increased significantly. However, the protein content in muscle had no significant difference among dietary treatments. The carbohydrate levels affected significantly the glycogen content in liver and muscle. The glycogen content in liver and muscle increased significantly when the carbohydrate levels were equal to or over 46% and 40%, respectively. The activities of intestinal trypsin and amylase decreased firstly, and then showed a tendency of increase, and lipase activity significantly increased. The content of serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), and glutamic-oxalacetic transaminase (GOT)/ glutamic-pyruvic transaminase (GPT)values had a rising trend with dietary carbohydrate levels increasing and protein level decreasing. These results indicated that lower protein (protein levels below 26%) and higher carbohydrate (carbohydrate level above 37%) imposed a negative effect on growth performance, and the hepatosmatic index, viscera index, intestinosomatic index and the fat con-tent of muscle and liver were significantly increased at high level of dietary carbohydrate.关键词
草鱼/蛋白质/碳水化合物/生长/肠道消化酶/血清指标Key words
Ctenopharyngodon idella/protein/carbohydrates/growth/intestinal digestive enzyme/serum indexes分类
农业科技引用本文复制引用
胡毅,陈云飞,张德洪,陈团,刘波,高启平..不同碳水化合物和蛋白质水平膨化饲料对大规格草鱼生长、肠道消化酶及血清指标的影响[J].水产学报,2018,42(5):777-786,10.基金项目
湖南省科技重大专项(2017NK1030) (2017NK1030)
通威股份有限公司产学研项目(TW2014A013) (TW2014A013)
国家星火计划重点项目(2011GA770007) (2011GA770007)
Major Science and Technology Special Project in Hunan,China(2017NK1030) (2017NK1030)
Production Project of Tongwei Co.,LTD.(TW2014A013) (TW2014A013)
National Spark Plan Key Project of China(2011GA770007) (2011GA770007)