水产学报2018，Vol.42Issue(5)：787-796,10.DOI:10.11964/jfc.20170410810
杜仲对草鱼生长、肌肉品质和胶原蛋白基因表达的影响
Effects of dietary Eucommia ulmoides on growth,flesh quality,and collagen gene expression of grass carp(Ctenopharyngodon idella)
摘要
Abstract
The present study was conducted to investigate the effects of dietary Eucommia ulmoides(EU)on growth performance,flesh quality,collagen genes COL1A1 and COL1A2 expression of grass carp(Ctenopharyn-godon idella).A total of 120 grass carp with an initial body weight of(215.0±0.4)g were randomly allocated into 2 groups with 3 replicates per group and 20 fish per replicate. The fish were fed formula diet without (control) or with the supplementation of 2% Eucommia ulmoides(EU)for 8 weeks.The supplementation of 2% EU did not significantly affect the growth performance of grass carp when compared with the control (P>0.05), but increased the collagen content in muscle, skin and liver (P<0.05). Total essential amino acids (TEAA), total non-essential amino acids (TNEAA) and total amino acids (TAA) in muscle of grass carp fed EU diet were significantly higher than those of control group. Fish fed EU showed significantly lower flesh thawing loss and centrifugal loss (higher water holding capacity) (P<0.05), but muscle fiber density and muscle fiber diameter showed no difference between the two groups (P>0.05). Moreover, dietary EU significantly promoted the relative expression level of COL1A1 and COL1A2 mRNA in muscle,skin at the 4thweek,the 8thweek,and the expression in liver at the 8th week (P<0.05). In conclusion, dietary EU could improve the flesh quality of large-size grass carp.关键词
草鱼/杜仲/生长/胶原蛋白/肌肉品质/COL1A1/COL1A2/基因表达Key words
Ctenopharyngodon idella/Eucommia ulmoides/growth/collagen/flesh quality/COL1A1/COL1A 2/gene expression分类
生物科学引用本文复制引用
许晓莹,李小勤,孙文通,姜维波,潘雯倩,汪军鹏,土志涵,冷向军..杜仲对草鱼生长、肌肉品质和胶原蛋白基因表达的影响[J].水产学报,2018,42(5):787-796,10.基金项目
国家自然科学基金(31772858) （31772858）
National Natural Science Foundation of China(31772858) （31772858）
