山东科学2018，Vol.31Issue(2)：14-20,7.DOI:10.3976/j.issn.1002-4026.2018.02.003
UHPLC-MS/MS法同时测定大鼠血浆中汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷含量
Simultaneous determination of wogonin, 4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma by UHPLC-MS/MS
摘要
Abstract
A rapid ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous analysis of wogonin,4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma.Apiin was selected as the internal standard.The analysis was carried out on an ACQUITY UPLC HSS T3 column (2.1 mmn× 100 mm,1.8 μm),with acetonitrile and 0.1％ formic acid aqueous solution,gradient elution.The flow rate was 0.2 mL · min-1.Linear responses were obtained for wogonin,4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide ranging from 1 to 1 000 ng· mL-1.The correlation coefficients were better than 0.99.Both recovery rates and matrix effects met the requirement of biological sample analysis.The established UHPLC-MS/MS quantitative analysis method shows high sensitivity,accuracy and satisfying recovery,and can be utilized to determine the pharmacokinetic data of wogonin,4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma.关键词
UHPLC-MS/MS/大鼠血浆/汉黄芩素/隐绿原酸/白杨素-7-O-葡萄糖醛酸苷Key words
UHPLC-MS/MS/rats plasma/wogonin/4-dicaffeoylquinic acid/chrysin-7-O-Beta-D-glucoronide分类
医药卫生引用本文复制引用
李翠,罗孟雄,聂其霞,臧琛,王国华,罗赣,张保献..UHPLC-MS/MS法同时测定大鼠血浆中汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷含量[J].山东科学,2018,31(2):14-20,7.基金项目
全国中药特色技术传承人才培训项目(L2016002) （L2016002）
