上海农业学报2018，Vol.34Issue(2)：8-13,6.DOI:10.15955/j.issn1000-3924.2018.02.02
不同培养基质和培养时间对蛹虫草子实体生物量和蛋白质氨基酸组成的影响
Effects of different culture media and culture time on biomass and protein amino acid composition of Cordyceps militaris fruiting body
摘要
Abstract
In order to provide reference for the full development and utilization of Cordyceps militaris,the effects of different culture media and culture time on the biomass and amino acid content and composition of fruiting body of C. militaris cultivated strain CM-H0810 were studied. The results showed that the biomass of fruiting body cultured by culture medium E (silkworm chrysalis)was the lowest,but the total amino acid content was the highest,and the ratio of essential amino acids to nonessential amino acids and the ratio of essential amino acids to total amino acids were ideal.Compared with culture medium C (rice),the biomass,protein content and total amino acids of fruiting body cultured by culture medium A (wheat kernel)were higher.After adding 10% silkworm chrysalis powder to culture medium A (wheat grain)or C (rice),the change of fruiting body biomass was not obvious,but the protein content and total amino acids were significantly higher than those of non silkworm chrysalis powder treatment.The highest biomass of fruiting body was obtained in the fifty-fifth day cultured by culture medium A (wheat kernel),and the highest amino acid content was obtained in the sixtieth day.Although Leu was the limiting amino acid of all the fruiting bodies of C.militaris cultured by different culture media,but the order of the amino acid coefficient was different. The amino acid coefficients of fruiting body samples with different growth time were all Thr>Phe+Tyr>Lys>Val>Ile>Leu>Met+Cys.关键词
蛹虫草/子实体生物量/氨基酸含量/培养基质/培养时间Key words
Cordyceps militaris/Biomass of fruiting body/Amino acid content/Medium/Culture time分类
农业科技引用本文复制引用
于荣利,高新华,张红霞,唐庆九..不同培养基质和培养时间对蛹虫草子实体生物量和蛋白质氨基酸组成的影响[J].上海农业学报,2018,34(2):8-13,6.基金项目
国家科技支撑计划课题"食用菌功能因子提取制备及产品开发与产业化"(2013BAD16B08-02) （2013BAD16B08-02）
