上海农业学报2018，Vol.34Issue(2)：18-22,5.DOI:10.15955/j.issn1000-3924.2018.02.04
MgO纳米颗粒对油菜子叶外植体的影响
Effects of MgO nanoparticles on the cotyledon explants from Brassica napus L. in vitro
何晓兰 1Marian Brestic 1郭书巧 2倪万潮1
作者信息
- 1. 江苏省农业科学院经济作物研究所,南京210014
- 2. 斯洛伐克农业大学植物生理学院,尼特拉市94976
- 折叠
摘要
Abstract
Magnesium oxide nanoparticles (MgO NPs)have wide uses in pharmaceuticals,toxic waste remediation,toxic gas removal,paint and semiconductors,but at present,there are not studies on plant growth and development with MgO NPs.This study investigated effects of MgO NPs on the cotyledons of rape(Brassica napus L.)variety'Ningyou 12'.The cotyledons of 4—5 d rape seedlings on MS media were cut and transferred to the rape adventitions bud regeneration media (MS+4.5 mg/L 6-BA)added 0—10.0 mmol/L MgO NPs for 21 d. Compared with negative control,the rate of adventitious buds was increased significantly on 1.5 mmol/L MgO NPs media from 36.67% to 83.33%,but inhibited obviously by MgO NPs with the concentration over 2.0 mmol/L, and the regeneration ability of cotyledons was suppressed completely by the MgO NPs over 8. 0 mmol/L. However,the adventitions bud regeneration rate and the number of adventitious buds each cotyledon were not obviously different between 2.0 mmol/L and 4.0 mmol/L MgO NPs,but the root regeneration rate and weight of cotyledon explants with 4.0 mmol/L MgO NPs treatment were better than that of 2.0 mmol/L.It was possible that aggregation effect of nanoparticles decreased the inhibition function on rape cotyledon explants. These results showed that low concentration of MgO NPs (1. 5 mmol/L)could increase the regeneration ability of rape cotyledon explants,but over 2.0 mmol/L,MgO NPs could cause toxic effects obviously.In addition,MgO NPs could be tried to use on the plant tissue culture as a kind of nontoxic and cheap additive.关键词
MgO纳米颗粒(MgONPs)/油菜子叶外植体/不定芽再生/无毒环保Key words
MgO nanoparticles(MgO NPs)/Rape cotyledon explants/Adventitions bud regeneration/Nontoxic and enviromental protection分类
农业科技引用本文复制引用
何晓兰,Marian Brestic,郭书巧,倪万潮..MgO纳米颗粒对油菜子叶外植体的影响[J].上海农业学报,2018,34(2):18-22,5.
