上海农业学报2018，Vol.34Issue(2)：28-32,5.DOI:10.15955/j.issn1000-3924.2018.02.06
淹涝处理下樱桃总RNA提取方法的建立与优化
Methods of extracting total RNA from cherry under waterlogging
摘要
Abstract
Total RNA was isolated from leaves and roots of cherry under waterlogging through CTAB method,TaKaRa MiniBEST Plant RNA Extraction Kit and improved Extraction Kit method.The quality of the total RNA was tested by gel electrophoresis and Ultramicro nucleic acid/protein detector.The results showed that total RNA extracted by improved TaKaRa MiniBEST Plant RNA Extraction Kit had two clear bands of 28S rRNA and 18S rRNA without degradation,and with higher purity and good integrity.Then,the total RNA of leaves and roots of cherry after 0—4 days waterlogging treatment were extracted by improved kit method,the quality of the RNA were determined by RT-PCR,the results showed that the PCR product had a distinct band with the same size as theoretical fragment and could satisfy the demands of molecular biology experiment. So the improved TaKaRa MiniBEST Plant RNA Extraction Kit method was the ideal method for the extraction of total RNA from cherry after waterlogging.关键词
樱桃/淹涝处理/总RNA/提取方法Key words
Cherry/Waterlogging treatment/Total RNA/Extraction method分类
农业科技引用本文复制引用
生利霞,孟祥毅,王猛,王倩,冯立国..淹涝处理下樱桃总RNA提取方法的建立与优化[J].上海农业学报,2018,34(2):28-32,5.基金项目
国家自然科学基金(31301746) （31301746）
