上海农业学报2018，Vol.34Issue(2)：39-42,4.DOI:10.15955/j.issn1000-3924.2018.02.08
小麦小孢子培养愈伤组织的高频诱导及其绿苗再生
The high-frequency callus induction and green plantlet regeneration under in vitro culture of wheat microspores
摘要
Abstract
The wheat variety 'Alondra' was used as a test material, its isolated spikes, isolated microspores and florets were pretreated via heat shock,low temperature,hormone,etc.,and the effects of those pretreatments on the callus yield were compared under in vitro culture of wheat microspores. The results showed that the callus yield of wheat microspores was significantly increased through 3-day low-temperature pretreatment of the florets at 5 ℃ and 2-day heat-shock treatment of the microspores at 33℃, and the green regenerative plantlets were finally formed through the calli differentiating.关键词
小麦/小孢子培养/预处理/愈伤组织诱导Key words
Wheat/Microspore culture/Pretreatment/Callus induction分类
农业科技引用本文复制引用
何婷,郭桂梅,陈志伟,张述伟,宗营杰,陆瑞菊,王亦菲,刘成洪..小麦小孢子培养愈伤组织的高频诱导及其绿苗再生[J].上海农业学报,2018,34(2):39-42,4.基金项目
中国科学院科技服务网络计划(KFJ-STS-ZDTP-002) （KFJ-STS-ZDTP-002）
上海市科技兴农重点攻关项目(应用基础类)(沪农科攻字2015第6-1-4号) （应用基础类）
