实验动物与比较医学2018，Vol.38Issue(2)：86-90,5.DOI:10.3969/j.issn.1674-5817.2018.02.002
鸭肠炎病毒感染诱导鸭胚成纤维细胞γ干扰素上调表达及初步机制的研究
Up-regulated Expression and Preliminary Mechanism of Interferon γ in Duck Embryonic Fibroblasts Induced by Duck Enteritis Virus
摘要
Abstract
Objective In order to investigate the mechanism of interferon γ (IFNγ) up-regulation in duck embryo fibroblasts (DEF) induced by duck enteritis virus(DEV) infection.Methods The total RNA was extracted from DEF at 24 h,36 h,48 h and 60 h after DEV infection,and reverse transcripted into cDNA.The expression of IFNγ and related interferon stimulated genes (ISGs) was detected by fluorescence quantitative PCR using specific primers.Results The expression level of IFNγwas significantly up-regulated at 24 h,36 h,48 h and 60 h,and the mRNA expression levels of Myxovirus resistance (Mx)、DEAD-box protein 50(DDX50)、2'-5' oligoadenylates synthesis L (OASL) were also up-regulated in DEV-infected cells compared with those of uninfected cells.Conclusions DEV-infected DEF cells were able to induce IFNγ production and up-regulation of related ISGs.关键词
鸭肠炎病毒(DEV)/鸭胚成纤维细胞(DEF)/γ干扰素(IFNγ)/干扰素刺激基因(ISGs)/上调表达Key words
Duck enteritis virus(DEV)/Duck embryo fibroblasts(DEF)/Interferon γ(IFNγ)/Interferon stimulated genes(ISGs)/Up-regulated expression分类
生物科学引用本文复制引用
李思琪,尹海畅,赵丽丽,王怡平,金建丽,陈洪岩..鸭肠炎病毒感染诱导鸭胚成纤维细胞γ干扰素上调表达及初步机制的研究[J].实验动物与比较医学,2018,38(2):86-90,5.基金项目
中央级公益性科研院所基本科研业务费专项(1610302016013,1610302017013) （1610302016013,1610302017013）
黑龙江省自然科学基金重点项目(ZD2016006) （ZD2016006）
中国农业科学院基本科研业务费专项(Y2016PT41) （Y2016PT41）
